{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T11:26:09Z","timestamp":1761823569083},"reference-count":59,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2013,9,15]],"date-time":"2013-09-15T00:00:00Z","timestamp":1379203200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Swine influenza virus (SIV) infections continue to cause production losses in the agricultural industry in addition to being a human public health concern. The primary method of controlling SIV is through vaccination. The killed SIV vaccines currently in use must be closely matched to the challenge virus, and their protective efficacy is limited. Live attenuated influenza vaccines (LAIV) provide strong, long-lived cell-mediated and humoral immunity against different influenza virus subtypes with no need for antigen matching. Here we report the generation of a new potential LAIV, an eight-segment SIV harboring two different SIV hemagglutinins (HAs), H1 and H3, in the genetic background of H1N1 SIV. This mutant SIV was generated by fusing the H3 HA ectodomain from A\/Swine\/Texas\/4199-2\/98 (H3N2) to the cytoplasmic tail, transmembrane domain, and stalk region of neuraminidase (NA) from A\/Swine\/Saskatchewan\/18789\/02 (H1N1) SIV. While this H1-H3 chimeric SIV, when propagated\n            <jats:italic>in vitro<\/jats:italic>\n            in the presence of exogenous neuraminidase, showed kinetics and growth properties similar to those of the parental wild-type virus,\n            <jats:italic>in vivo<\/jats:italic>\n            it was highly attenuated in pigs, demonstrating a great potential for serving as a dual LAIV. Furthermore, vaccination with the H1-H3 virus elicited robust immune responses, which conferred complete protection against infections with both H1 and H3 SIV subtypes in pigs.\n          <\/jats:p>","DOI":"10.1128\/jvi.01348-13","type":"journal-article","created":{"date-parts":[[2013,7,11]],"date-time":"2013-07-11T04:35:23Z","timestamp":1373517323000},"page":"10114-10125","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["An Eight-Segment Swine Influenza Virus Harboring H1 and H3 Hemagglutinins Is Attenuated and Protective against H1N1 and H3N2 Subtypes in Pigs"],"prefix":"10.1128","volume":"87","author":[{"given":"Aleksandar","family":"Masic","sequence":"first","affiliation":[{"name":"Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyun-Mi","family":"Pyo","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shawn","family":"Babiuk","sequence":"additional","affiliation":[{"name":"National Centre for Foreign Animal Disease\u2014Canadian Food Inspection Agency, Winnipeg, MB, Canada"},{"name":"Department of Immunology, University of Manitoba, Winnipeg, MB, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"Vaccine and Infectious Disease Organization, University of Saskatchewan, Saskatoon, SK, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Diseases of swine","author":"Straw BE","year":"1999","unstructured":"StrawBE D'AllaireS MenegelingWL TaylorDJ. 1999. 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