{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T01:22:17Z","timestamp":1788225737170,"version":"build-2803163510"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,17]],"date-time":"2026-02-17T00:00:00Z","timestamp":1771286400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2026,2,17]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Powassan virus (POWV) is a tick-borne flavivirus endemic to the United States, Canada, and parts of Russia. POWV remains an under-studied pathogen, despite the potential for serious and life-threatening neurologic complications following infection. While prior studies have characterized viral diversity due to single nucleotide polymorphisms, little is known about POWV recombination, defective RNAs (D-RNAs), and functional structural variants (SVs). Understanding POWV recombination in its natural vector can provide important insights into its replication and evolution. We analyzed POWV sequence data from 53 ticks collected from the Northeastern United States to characterize and quantify recombination patterns in naturally infected ticks. We then compared these results to single-passage isolates. Deletions were common in POWV RNA from ticks, and several areas of the genome were enriched for recombination junctions. Deletions were often associated with areas of microhomology. While most deletions were sample-specific, two major deletion archetypes were observed across multiple tick samples. The first consisted of small 19\u201350 base deletions in the methyltransferase domain of the ns5 RNA-dependent RNA-polymerase coding sequence, resulting in a mixture of putative SVs and D-RNAs. The second consisted of approximately 1,600 base deletions spanning the ns2a-ns3 coding sequences, resulting in putative D-RNAs with abrogated viral protease function. Deletions in ns2a-ns3 were significantly enriched after one passage in baby hamster kidney cells, despite a decrease in overall deletions. These results demonstrate that POWV RNA recombines frequently, with certain variants more common than others. These findings may carry implications for virus immune evasion and persistence in ticks.<\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>Powassan virus is a tick-borne flavivirus that can cause serious, life-threatening neurological disease. Understanding how Powassan virus replicates and evolves within its tick vector may elucidate factors important for persistence, transmission, and human disease. Defective RNAs (D-RNAs) are replication-incompetent viral genomes generated through internal deletions. D-RNAs are associated with disease severity and persistent infection in other viruses but have not been described for Powassan virus. Here, we show that Powassan virus produces abundant putative D-RNAs in field-collected ticks and that patterns of D-RNA expression change after one passage in mammalian cells. Although the function of these D-RNAs remains unknown, this work demonstrates that they occur under natural conditions and establishes a critical framework for investigating the role of D-RNAs in Powassan virus replication and transmission.<\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.01356-25","type":"journal-article","created":{"date-parts":[[2026,1,21]],"date-time":"2026-01-21T14:35:02Z","timestamp":1769006102000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":3,"title":["Evidence for Powassan virus deletions and defective RNA in field-collected ticks"],"prefix":"10.1128","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9277-2937","authenticated-orcid":true,"given":"Samantha J.","family":"Courtney","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03k1gpj17","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Vector-borne Infectious Diseases, Colorado State University","place":["Fort Collins, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9008-425X","authenticated-orcid":false,"given":"Rose M.","family":"Langsjoen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/018rbev86","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Laboratory Medicine, Emory University","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chasity E.","family":"Trammell","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03k1gpj17","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Vector-borne Infectious Diseases, Colorado State University","place":["Fort Collins, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca M.","family":"Robich","sequence":"additional","affiliation":[{"name":"MaineHealth Institute for Research, Maine Medical Center","place":["Scarborough, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5145-9047","authenticated-orcid":true,"given":"Heidi K.","family":"Goethert","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05wvpxv85","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Disease and Global Health, Tufts University","place":["North Grafton, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah J.","family":"McMinn","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03k1gpj17","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Vector-borne Infectious Diseases, Colorado State University","place":["Fort Collins, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4548-8663","authenticated-orcid":true,"suffix":"III","given":"Sam R.","family":"Telford","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05wvpxv85","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Infectious Disease and Global Health, Tufts University","place":["North Grafton, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4458-9959","authenticated-orcid":true,"given":"Gregory D.","family":"Ebel","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03k1gpj17","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Vector-borne Infectious Diseases, Colorado State University","place":["Fort Collins, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5942-1534","authenticated-orcid":true,"given":"Anne","family":"Piantadosi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/018rbev86","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Pathology and Laboratory Medicine, Emory University","place":["Atlanta, USA"]},{"id":[{"id":"https:\/\/ror.org\/018rbev86","id-type":"ROR","asserted-by":"publisher"}],"name":"Division of Infectious Diseases, Department of Medicine, Emory University","place":["Atlanta, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-112408-085446"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1973.22.78"},{"key":"e_1_3_4_4_2","first-page":"708","article-title":"Powassan virus: isolation of virus from a fatal case of encephalitis","volume":"80","author":"McLean DM","year":"1959","unstructured":"McLean DM, Donohue WL. 1959. 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