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While human-to-human transmission is limited, there is a risk that these zoonotic viruses may acquire adaptive mutations enabling them to propagate efficiently and cause devastating human pandemics. Therefore, it is important to identify viral determinants that provide these viruses with a replicative advantage in human cells. Here, we tested the growth of influenza A virus in a subset of human cell lines and found that abortive replication of H1N1 viruses in HeLa cells can be circumvented upon the introduction of H5N1 virus HA and NP. Overall, this work leverages the genetic diversity of multiple human cell lines to highlight viral determinants that could contribute to H5N1 virus pathogenesis and tropism.<\/jats:p>","DOI":"10.1128\/jvi.01410-19","type":"journal-article","created":{"date-parts":[[2019,11,26]],"date-time":"2019-11-26T11:11:51Z","timestamp":1574766711000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Viral Determinants in H5N1 Influenza A Virus Enable Productive Infection of HeLa Cells"],"prefix":"10.1128","volume":"94","author":[{"given":"Ariel","family":"Rodriguez-Frandsen","sequence":"first","affiliation":[{"name":"Infectious and Inflammatory Diseases Center, Sanford Burnham Prebys Medical Discovery Institute, La Jolla, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura","family":"Martin-Sancho","sequence":"additional","affiliation":[{"name":"Infectious and Inflammatory Diseases 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