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Anti-CD81 antibodies and siRNA-CD81 silencing markedly inhibited (&gt;90%) HCVser infection irrespective of HCV genotype, viral load, or liver donor, while hCD81-large intracellular loop (LEL) had no effect. However, JFH1\/HCVcc infection of hepatocytes was modestly inhibited (40 to 60%) by both hCD81-LEL and anti-CD81 antibodies. In conclusion, CD81 is involved in HCVser infection of human hepatocytes, and comparative studies of HCVser versus JFH1\/HCVcc infection of human hepatocytes and Huh-7.5 cells revealed that the cell-virion combination is determinant of the entry process.<\/jats:p>","DOI":"10.1128\/jvi.01443-07","type":"journal-article","created":{"date-parts":[[2007,10,18]],"date-time":"2007-10-18T01:04:48Z","timestamp":1192669488000},"page":"569-574","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":147,"title":["Serum-Derived Hepatitis C Virus Infection of Primary Human Hepatocytes Is Tetraspanin CD81 Dependent"],"prefix":"10.1128","volume":"82","author":[{"given":"Sonia","family":"Molina","sequence":"first","affiliation":[{"name":"Inserm, U632, 34293 Montpellier, France; Univ. 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