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However, this model relies on strains of MCMV that have been serially passaged in the laboratory for over four decades. These laboratory strains have been cloned as bacterial artificial chromosomes (BACs), which permits rapid and precise manipulation. Low-passage strains of MCMV add to the utility of the mouse model of HCMV infection but do not exist as cloned BACs. This study describes the first such low-passage MCMV BAC. This BAC-derived G4 was initially attenuated\n                    <jats:italic>in vivo<\/jats:italic>\n                    , with subsequent full genomic sequencing revealing a novel spliced transcript required for salivary gland tropism. These data suggest that MCMV, like HCMV, undergoes tissue culture adaptation that can limit\n                    <jats:italic>in vivo<\/jats:italic>\n                    growth and supports the use of BAC clones as a way of standardizing viral strains and minimizing interlaboratory strain variation.\n                  <\/jats:p>","DOI":"10.1128\/jvi.01456-20","type":"journal-article","created":{"date-parts":[[2020,8,26]],"date-time":"2020-08-26T10:18:25Z","timestamp":1598437105000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Repair of an Attenuated Low-Passage Murine Cytomegalovirus Bacterial Artificial Chromosome Identifies a Novel Spliced Gene Essential for Salivary Gland Tropism"],"prefix":"10.1128","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8601-8292","authenticated-orcid":false,"given":"Alec James","family":"Redwood","sequence":"first","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia, Crawley, Western Australia, Australia"},{"name":"The Institute for Respiratory Health, Nedlands, Western Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura Lee","family":"Masters","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia, Crawley, Western Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baca","family":"Chan","sequence":"additional","affiliation":[{"name":"School of Biomedical Sciences, University of Western Australia, Crawley, Western Australia, Australia"},{"name":"The Institute for Respiratory Health, Nedlands, Western Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shay","family":"Leary","sequence":"additional","affiliation":[{"name":"The Institute for Immunology and Infectious Diseases, Murdoch University, Murdoch, Western Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cathy","family":"Forbes","sequence":"additional","affiliation":[{"name":"Telethon Kids Institute, Perth Children's Hospital, Nedlands, Western Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stipan","family":"Jonji\u0107","sequence":"additional","affiliation":[{"name":"The Department for Histology and Embryology and Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanda","family":"Jurani\u0107 Lisni\u0107","sequence":"additional","affiliation":[{"name":"The Department for Histology and Embryology and Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Berislav","family":"Lisni\u0107","sequence":"additional","affiliation":[{"name":"The Department for Histology and Embryology and Center for Proteomics, Faculty of Medicine, University of Rijeka, Rijeka, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lee Martin","family":"Smith","sequence":"additional","affiliation":[{"name":"SpeedX Pty Ltd., National Innovative Centre, Evenleigh, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.2034"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365549950163969"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.1.86-124.1997"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2011.04.010"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/v10120693"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-012369454-6\/50029-7"},{"key":"e_1_3_3_8_2","first-page":"23","volume-title":"Cytomegalovirus: from molecular pathogenesis to intervention","author":"Redwood A","year":"2013","unstructured":"Redwood A, Shellam G, Smith L. 2013. 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