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HAdV26 has emerged as a promising candidate vector for development of vaccines due to its relatively low seroprevalence and its ability to induce potent immune responses against inserted transgenes. However, data regarding the basic biology of the virus, like receptor usage or intracellular trafficking, are limited. In this work, we found that efficient infection of human epithelial cell lines by HAdV26 requires the expression of the \u03b1v\u03b23 integrin. By studying intracellular trafficking of fluorescently labeled HAdV26 in a cell clone with stably increased expression of \u03b1v\u03b23 integrin, we observed that HAdV26 colocalizes with \u03b1v\u03b23 integrin and confirmed that \u03b1v\u03b23 integrin expression facilitates efficient HAdV26 internalization. These results will allow further improvement of HAdV26-based vectors for gene transfer and vaccination.<\/jats:p>","DOI":"10.1128\/jvi.01474-18","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T05:28:43Z","timestamp":1539581323000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["\u03b1v\u03b23 Integrin Is Required for Efficient Infection of Epithelial Cells with Human Adenovirus Type 26"],"prefix":"10.1128","volume":"93","author":[{"given":"Davor","family":"Nesti\u0107","sequence":"first","affiliation":[{"name":"Laboratory for Cell Biology and Signalling, Division of Molecular Biology, Ru\u0111er Bo\u0161kovi\u0107 Institute, Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taco G.","family":"Uil","sequence":"additional","affiliation":[{"name":"Viral Vaccine Discovery and Early Development, Janssen Vaccines and Prevention BV, Leiden, 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