{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:02:19Z","timestamp":1783465339666,"version":"3.55.0"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2010,12,15]],"date-time":"2010-12-15T00:00:00Z","timestamp":1292371200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>RNA-seq provides a rich source of transcriptome information with high qualitative and quantitative value. Here, we provide a pipeline for Epstein-Barr virus (EBV) transcriptome analysis using RNA-seq and we apply it to two type I latency cell lines, Mutu I and Akata. This analysis revealed substantial average expression levels of many lytic genes in predominantly latent cell populations. The lytic transcripts BHLF1 and LF3 were expressed at levels greater than those for 98% of all cellular polyadenylated transcripts. Exon junction mapping accurately identified the Qp-derived EBNA1 splicing pattern, lytic gene splicing, and a complex splicing pattern within the BamHI A region.<\/jats:p>","DOI":"10.1128\/jvi.01521-10","type":"journal-article","created":{"date-parts":[[2010,10,14]],"date-time":"2010-10-14T07:21:57Z","timestamp":1287040917000},"page":"13053-13058","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Quantitative and Qualitative RNA-Seq-Based Evaluation of Epstein-Barr Virus Transcription in Type I Latency Burkitt's Lymphoma Cells"],"prefix":"10.1128","volume":"84","author":[{"given":"Zhen","family":"Lin","sequence":"first","affiliation":[{"name":"Department of Pathology, Tulane University Health Sciences Center and Tulane Cancer Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guorong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Computer Science, University of New Orleans, Lakefront, 2000 Lakeshore Drive, New Orleans, Louisiana 70148"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Computer Science, University of New Orleans, Lakefront, 2000 Lakeshore Drive, New Orleans, Louisiana 70148"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Taylor","sequence":"additional","affiliation":[{"name":"Department of Computer Science, University of New Orleans, Lakefront, 2000 Lakeshore Drive, New Orleans, Louisiana 70148"},{"name":"Research Institute for Children, Children's Hospital, New Orleans, 200 Henry Clay Avenue, New Orleans, Louisiana 70118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongxiao","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Computer Science, University of New Orleans, Lakefront, 2000 Lakeshore Drive, New Orleans, Louisiana 70148"},{"name":"Research Institute for Children, Children's Hospital, New Orleans, 200 Henry Clay Avenue, New Orleans, Louisiana 70118"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik K.","family":"Flemington","sequence":"additional","affiliation":[{"name":"Department of Pathology, Tulane University Health Sciences Center and Tulane Cancer Center, 1430 Tulane Avenue, New Orleans, Louisiana 70112"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1743-422X-3-43"},{"key":"e_1_3_2_3_2","first-page":"853916","volume":"2010","year":"2010","unstructured":"Costa, V., C. 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