{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:50:25Z","timestamp":1793728225472,"version":"4.6.1"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T00:00:00Z","timestamp":1579219200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T00:00:00Z","timestamp":1579219200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Houston Zoo"},{"DOI":"10.13039\/100014974","name":"International Elephant Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100014974","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000072","name":"HHS | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["Intramural Research Program grant"],"award-info":[{"award-number":["Intramural Research Program grant"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2020,1,17]]},"abstract":"<jats:p>Whether clinical illness and deaths associated with elephant endotheliotropic herpesvirus (EEHV) infection result from primary infection or reactivation of latent virus is a longstanding question in the field. By applying a relatively new assay, the luciferase immunoprecipitation system (LIPS), combined with the genomic sequences of the viruses, we gained the insights and tools needed to resolve this issue. Our EEHV1-specific LIPS assay should be useful for assessing the vulnerability of elephant calves to infection with different EEHVs and evaluating antibody responses to anti-EEHV vaccines. A significant proportion of the Asian elephant population is under some form of human care. Hence, the ability to screen for EEHV immune status in elephant calves should have a major impact on the management of these animals worldwide.<\/jats:p>","DOI":"10.1128\/jvi.01528-19","type":"journal-article","created":{"date-parts":[[2019,11,12]],"date-time":"2019-11-12T11:09:38Z","timestamp":1573556978000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Lethal Hemorrhagic Disease and Clinical Illness Associated with Elephant Endotheliotropic Herpesvirus 1 Are Caused by Primary Infection: Implications for the Detection of Diagnostic Proteins"],"prefix":"10.1128","volume":"94","author":[{"given":"Angela","family":"Fuery","sequence":"first","affiliation":[{"name":"Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor","family":"Pursell","sequence":"additional","affiliation":[{"name":"Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Tan","sequence":"additional","affiliation":[{"name":"Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongsheng","family":"Peng","sequence":"additional","affiliation":[{"name":"Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter D.","family":"Burbelo","sequence":"additional","affiliation":[{"name":"Dental Clinical Research Core, NIDCR, NIH, Bethesda, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary S.","family":"Hayward","sequence":"additional","affiliation":[{"name":"Viral Oncology Program, Johns Hopkins School of Medicine, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Ling","sequence":"additional","affiliation":[{"name":"Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/ilar\/ilv041"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0202438"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1638\/1042-7260-44.1.100"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep06299"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1638\/2016-0063R.1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01951-17"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2013.03.008"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12917-015-0522-6"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12985-019-1142-8"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6750-5-22"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00674-15"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00250-14"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00476-09"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00350-08"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00474-08"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.06.013"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1638\/1042-7260-44.1.136"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1638\/2015-0072.1"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1638\/2015-0074.1"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2012.07.024"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1638\/1042-7260-44.1.42"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.71.8.925"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1039\/c0mb00342e"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0022576"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jim.2017.02.001"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0160192"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00106-13"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00081-15"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.3791\/1549"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0116318"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01673-14"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01528-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01528-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:02:03Z","timestamp":1646492523000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01528-19"}},"subtitle":[],"editor":[{"given":"Rozanne M.","family":"Sandri-Goldin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,1,17]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2020,1,17]]}},"alternative-id":["10.1128\/JVI.01528-19"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01528-19","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,17]]},"assertion":[{"value":"2019-09-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01528-19"}}