{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T04:11:39Z","timestamp":1790395899719,"version":"4.1.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2020,2,14]],"date-time":"2020-02-14T00:00:00Z","timestamp":1581638400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018R1A2B2005390"],"award-info":[{"award-number":["2018R1A2B2005390"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["2017R1C1B2004989"],"award-info":[{"award-number":["2017R1C1B2004989"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2020,2,14]]},"abstract":"<jats:p>PACSIN2 is a lipid-binding protein that triggers the tubulation of the phosphatidic acid-containing membranes. The functional involvement of PACSIN2 in the virus life cycle has not yet been demonstrated. We showed that phosphorylation of PACSIN2 displayed a negative effect on NS5A and core interaction. The most significant finding is that NS5A prevents PKC\u03b1 from binding to PACSIN2. Therefore, the phosphorylation level of PACSIN2 is decreased in HCV-infected cells. We showed that HCV NS5A interrupted PKC\u03b1-mediated PACSIN2 phosphorylation at serine 313, thereby promoting NS5A-PACSIN2 interaction. We further demonstrated that PACSIN2 modulated lipid droplet formation through ERK1\/2 phosphorylation. These data provide evidence that PACSIN2 is a proviral cellular factor required for viral propagation.<\/jats:p>","DOI":"10.1128\/jvi.01531-19","type":"journal-article","created":{"date-parts":[[2019,12,4]],"date-time":"2019-12-04T13:22:25Z","timestamp":1575465745000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["PACSIN2 Interacts with Nonstructural Protein 5A and Regulates Hepatitis C Virus Assembly"],"prefix":"10.1128","volume":"94","author":[{"given":"Lap P.","family":"Nguyen","sequence":"first","affiliation":[{"name":"Laboratory of RNA Viral Diseases, Korea Zoonosis Research Institute, Chonbuk National University, Iksan, South Korea"},{"name":"National Research Laboratory of Hepatitis C Virus and Ilsong Institute of Life Science, Hallym University, Anyang, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si C.","family":"Tran","sequence":"additional","affiliation":[{"name":"National Research Laboratory of Hepatitis C Virus and Ilsong Institute of Life Science, Hallym University, Anyang, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiro","family":"Suetsugu","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Medicine and Cell Biology, Graduate School of Biosciences, Nara Institute of Science and Technology, Ikoma, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Sook","family":"Lim","sequence":"additional","affiliation":[{"name":"Laboratory of RNA Viral Diseases, Korea Zoonosis Research Institute, Chonbuk National University, Iksan, South Korea"},{"name":"National Research Laboratory of Hepatitis C Virus and Ilsong Institute of Life Science, Hallym University, Anyang, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon B.","family":"Hwang","sequence":"additional","affiliation":[{"name":"Laboratory of RNA Viral Diseases, Korea Zoonosis Research Institute, Chonbuk National University, Iksan, South Korea"},{"name":"National Research Laboratory of Hepatitis C Virus and Ilsong Institute of Life Science, Hallym University, Anyang, South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2019.01.001"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/v7112898"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1002032"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2018.02.016"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-43783-6"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2016.07.012"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-33861-6"},{"key":"e_1_3_2_9_2","first-page":"267","volume-title":"Hepatitis C viruses: genomes and molecular biology","author":"He Y","year":"2006","unstructured":"He Y, Staschke KA, Tan SL. 2006. HCV NS5A: a multifunctional regulator of cellular pathways and virus replication, p 267\u2013292. In Tan SL (ed), Hepatitis C viruses: genomes and molecular biology. Horizon Bioscience, Norfolk, UK."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111289200"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.2667"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jproteome.9b00130"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006834"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510727103"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1268"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00826-08"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2010.05.008"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1003478107"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1801849115"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1808821115"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006070"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.391078"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.167775"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3046"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01707-15"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.4161\/cc.10.1.14243"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.201601265R"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.080630"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.194472"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00059-11"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01660-14"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.85.217"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2014.11.003"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0156996"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-32216-5"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.02941"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.2337\/db15-1024"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2014.03.008"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800696200"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0064605"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.53.5.1261"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.152.5.1071"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08872-0"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2010.02.058"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.18801-0"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06001-11"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000035"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.009332-0"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M301430200"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01678-14"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.03353-12"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.084319"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-59745-394-3_24"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aje.a118408"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01662-16"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01240-18"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01531-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01531-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:04:00Z","timestamp":1646492640000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01531-19"}},"subtitle":[],"editor":[{"given":"J.-H.","family":"James Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,2,14]]},"references-count":55,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,2,14]]}},"alternative-id":["10.1128\/JVI.01531-19"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01531-19","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,2,14]]},"assertion":[{"value":"2019-09-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-11-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-02-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01531-19"}}