{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:56:47Z","timestamp":1791255407098,"version":"4.1.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2012,10,15]],"date-time":"2012-10-15T00:00:00Z","timestamp":1350259200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2012,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Rift Valley fever virus (RVFV) is a highly pathogenic<jats:named-content content-type=\"genus-species\">Phlebovirus<\/jats:named-content>that infects humans and ruminants. Initially confined to Africa, RVFV has spread outside Africa and presently represents a high risk to other geographic regions. It is responsible for high fatality rates in sheep and cattle. In humans, RVFV can induce hepatitis, encephalitis, retinitis, or fatal hemorrhagic fever. The nonstructural NSs protein that is the major virulence factor is found in the nuclei of infected cells where it associates with cellular transcription factors and cofactors. In previous work, we have shown that NSs interacts with the promoter region of the beta interferon gene abnormally maintaining the promoter in a repressed state. In this work, we performed a genome-wide analysis of the interactions between NSs and the host genome using a genome-wide chromatin immunoprecipitation combined with promoter sequence microarray, the ChIP-on-chip technique. Several cellular promoter regions were identified as significantly interacting with NSs, and the establishment of NSs interactions with these regions was often found linked to deregulation of expression of the corresponding genes. Among annotated NSs-interacting genes were present not only genes regulating innate immunity and inflammation but also genes regulating cellular pathways that have not yet been identified as targeted by RVFV. Several of these pathways, such as cell adhesion, axonal guidance, development, and coagulation were closely related to RVFV-induced disorders. In particular, we show in this work that NSs targeted and modified the expression of genes coding for coagulation factors, demonstrating for the first time that this hemorrhagic virus impairs the host coagulation cascade at the transcriptional level.<\/jats:p>","DOI":"10.1128\/jvi.01549-12","type":"journal-article","created":{"date-parts":[[2012,8,16]],"date-time":"2012-08-16T02:02:53Z","timestamp":1345082573000},"page":"11333-11344","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Large-Scale Chromatin Immunoprecipitation with Promoter Sequence Microarray Analysis of the Interaction of the NSs Protein of Rift Valley Fever Virus with Regulatory DNA Regions of the Host Genome"],"prefix":"10.1128","volume":"86","author":[{"given":"Rima","family":"Benferhat","sequence":"first","affiliation":[{"name":"Unit\u00e9 de G\u00e9n\u00e9tique Mol\u00e9culaire des Bunyavirus, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibaut","family":"Josse","sequence":"additional","affiliation":[{"name":"CNRS FRE3235 G\u00e9n\u00e9tique Mol\u00e9culaire et D\u00e9fense Antivirale, Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Albaud","sequence":"additional","affiliation":[{"name":"Institut Curie, Departement of Translational Research, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Gentien","sequence":"additional","affiliation":[{"name":"Institut Curie, Departement of Translational Research, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeyni","family":"Mansuroglu","sequence":"additional","affiliation":[{"name":"CNRS FRE3235 G\u00e9n\u00e9tique Mol\u00e9culaire et D\u00e9fense Antivirale, Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasco","family":"Marcato","sequence":"additional","affiliation":[{"name":"CNRS FRE3235 G\u00e9n\u00e9tique Mol\u00e9culaire et D\u00e9fense Antivirale, Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvie","family":"Sou\u00e8s","sequence":"additional","affiliation":[{"name":"CNRS FRE3235 G\u00e9n\u00e9tique Mol\u00e9culaire et D\u00e9fense Antivirale, Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Le Bonniec","sequence":"additional","affiliation":[{"name":"INSERM UMR S765 Thrombose: Epid\u00e9miologie, Physiopathologie et Th\u00e9rapeutiques Innovantes, Universit\u00e9 Paris Descartes, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mich\u00e8le","family":"Bouloy","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de G\u00e9n\u00e9tique Mol\u00e9culaire des Bunyavirus, Institut Pasteur, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eliette","family":"Bonnefoy","sequence":"additional","affiliation":[{"name":"CNRS FRE3235 G\u00e9n\u00e9tique Mol\u00e9culaire et D\u00e9fense Antivirale, Universit\u00e9 Paris Descartes, Sorbonne Paris Cit\u00e9, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.9.3565-3581.2006"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100208200"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2009.08.002"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000493"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.6.1961-1967.2003"},{"key":"e_1_3_3_7_2","first-page":"11","article-title":"The pathology of Rift Valley fever. 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