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Currently, vaccination is still the best way to prevent the prevalence of IBDV. However, with the occurrence of increasing numbers of variant IBDV strains, it is challenging to develop antigen-matched live attenuated vaccine. Here, we first developed a dual-promoter reverse-genetic system for the rapid generation of IBDV. Using this system, the attenuated IBDV\/1FLAG expressing VP1-FLAG, which displays the decreased viral RNP activity, was rescued. Moreover, IBDV\/1FLAG inoculation induced a similar level of neutralizing antibodies to that of its parental counterpart, protecting chickens against lethal challenge. Our study, for the first time, describes a dual-promoter reverse-genetic approach for the rapid generation of attenuated IBDV while maintaining entire parental antigenicity, suggesting a potential new method to attenuate epidemic variant IBDV strains for vaccine development.<\/jats:p>","DOI":"10.1128\/jvi.01569-19","type":"journal-article","created":{"date-parts":[[2020,1,9]],"date-time":"2020-01-09T14:06:41Z","timestamp":1578578801000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Rapid Generation of Attenuated Infectious Bursal Disease Virus from Dual-Promoter Plasmids by Reduction of Viral Ribonucleoprotein Activity"],"prefix":"10.1128","volume":"94","author":[{"given":"Hui","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, College of Animal Science and Technology, Zhejiang A&amp;F University, Hangzhou, Zhejiang Province, 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