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Although being extensively analyzed and dissected for decades, several facets of MLV biology are still poorly understood. One of the primary challenges has been enumerating total intact virus particles in a sample. While several analytical methods can precisely measure virus protein amounts, MLVs are known to induce the secretion of soluble and vesicle-associated viral proteins that can skew these measurements. With recent technological advances in flow cytometry, it is now possible to analyze viruses down to 90\u2009nm in diameter with an approach called flow virometry. The technique has the added benefit of being able to discriminate viruses from extracellular vesicles and free viral proteins in order to confidently provide an intact viral particle count. Here, we used flow virometry to provide new insights into the basic characteristics of Moloney MLV.<\/jats:p>","DOI":"10.1128\/jvi.01600-19","type":"journal-article","created":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T13:51:40Z","timestamp":1572616300000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Intact Viral Particle Counts Measured by Flow Virometry Provide Insight into the Infectivity and Genome Packaging Efficiency of Moloney Murine Leukemia Virus"],"prefix":"10.1128","volume":"94","author":[{"given":"Tyler Milston","family":"Renner","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera A.","family":"Tang","sequence":"additional","affiliation":[{"name":"University of Ottawa Flow Cytometry and Virometry Core Facility, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dylan","family":"Burger","sequence":"additional","affiliation":[{"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4652-3029","authenticated-orcid":false,"given":"Marc-Andr\u00e9","family":"Langlois","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Microbiology and Immunology, Faculty of Medicine, University of Ottawa, Ottawa, Ontario, Canada"},{"name":"University of Ottawa Flow Cytometry and Virometry Core Facility, Ottawa, Ontario, Canada"},{"name":"uOttawa Center for Infection, Immunity and Inflammation (CI3), Ottawa, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"933","article-title":"Biological studies on a lymphoid-leukemia virus extracted from sarcoma 37. 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