{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T12:59:45Z","timestamp":1790773185286,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2010,3,15]],"date-time":"2010-03-15T00:00:00Z","timestamp":1268611200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Burkitt lymphoma (BL) is etiologically associated with Epstein-Barr virus (EBV). EBV-positive BL tumors display two latent forms of infection. One is referred to as latency I infection, in which EBV expresses the virus genome maintenance protein EBNA1 as the only viral protein. The other is referred to as Wp-restricted latency and was recently identified in a subset of BL tumors. In these tumors, EBV expresses EBNA1, EBNA3A, EBNA3B, EBNA3C, a truncated form of EBNA-LP, and the viral Bcl-2 homologue BHRF1, all of which are driven by the BamHI W promoter (Wp). To investigate the role of EBV in Wp-restricted BL, we conditionally expressed a dominant-negative EBNA1 (dnEBNA1) mutant which interrupts the virus genome maintenance function of EBNA1 in the P3HR-1 BL cell line. Induction of dnEBNA1 expression caused loss of the EBV genome and resulted in apoptosis of P3HR-1 cells in the absence of exogenous apoptosis inducers, indicating that P3HR-1 cells cannot survive without EBV. Stable transfection of the BHRF1 gene into P3HR-1 cells rescued the cells from the apoptosis induced by dnEBNA1 expression, whereas stable transfection of truncated EBNA-LP, EBNA3A, or EBNA3C did not. Moreover, knockdown of BHRF1 expression in P3HR-1 cells resulted in increased cell death. These results indicate that EBV is essential for the survival of P3HR-1 cells and that BHRF1 functions as a survival factor. Our finding implies a critical contribution of BHRF1 to the pathogenesis of Wp-restricted BLs.<\/jats:p>","DOI":"10.1128\/jvi.01616-09","type":"journal-article","created":{"date-parts":[[2009,12,31]],"date-time":"2009-12-31T02:43:48Z","timestamp":1262227428000},"page":"2893-2901","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Epstein-Barr Virus-Encoded Bcl-2 Homologue Functions as a Survival Factor in Wp-Restricted Burkitt Lymphoma Cell Line P3HR-1"],"prefix":"10.1128","volume":"84","author":[{"given":"Ami","family":"Watanabe","sequence":"first","affiliation":[{"name":"Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo 060-0815, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiji","family":"Maruo","sequence":"additional","affiliation":[{"name":"Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo 060-0815, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taku","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo 060-0815, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo 060-0815, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi Ricardo","family":"Katsumura","sequence":"additional","affiliation":[{"name":"Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo 060-0815, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenzo","family":"Takada","sequence":"additional","affiliation":[{"name":"Department of Tumor Virology, Institute for Genetic Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo 060-0815, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210668"},{"key":"e_1_3_2_3_2","first-page":"1915","volume":"6","year":"1991","unstructured":"Askew, D. S., R. A. Ashmun, B. C. Simmons, and J. L. Cleveland. 1991. Constitutive c-myc expression in an IL-3-dependent myeloid cell line suppresses cell cycle arrest and accelerates apoptosis. Oncogene6:1915-1922.","journal-title":"Oncogene"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.85.11.3678"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/359552a0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.43.3.952-968.1982"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2006.07.014"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90123-T"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.10.8392-8395.1998"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/359554a0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02435-06"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.12.5547"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.9.6611-6618.1997"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.18.8479"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/295160a0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(196811)22:6<1089::AID-CNCR2820220603>3.0.CO;2-2"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.98.4.1865"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.211556598"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.012831-0"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm758"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.16.10709-10717.2005"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000341"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509988103"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2336099100"},{"key":"e_1_3_2_25_2","first-page":"2603","year":"2007","unstructured":"Kieff, E., and A. B. Rickinson. 2007. Epstein-Barr virus and its replication, p. 2603-2654. In D. M. Knipe and P. M. Howley (ed.), Fields virology, 5th ed. Lippincott Williams and Wilkins, Philadelphia, PA.","journal-title":"Fields virology"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.71.3.1766-1775.1997"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90449-X"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.12.9827-9831.1999"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.11.9150-9156.1998"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.3.1561-1564.2001"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.8.1183"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01096-06"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.5.3349"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.12.6826-6837.1991"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0604919104"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.19.10437-10447.2003"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/21.5.954"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2004.12.017"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(87)90055-9"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.44.3.834-844.1982"},{"key":"e_1_3_2_41_2","first-page":"2655","year":"2007","unstructured":"Rickinson, A. B., and E. Kieff. 2007. Epstein-Barr virus, p. 2655-2700. In D. M. Knipe and P. M. Howley (ed.), Fields virology, 5th ed. Lippincott Williams and Wilkins, Philadelphia, PA.","journal-title":"Fields virology"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.3.1651"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.02.029"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2005.tb03743.x"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.9.6069-6073.1994"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/348331a0"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.4.2014-2025.1993"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00579-07"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.7.4298-4307.2005"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-07-2573"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01616-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01616-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T13:55:37Z","timestamp":1646488537000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01616-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,3,15]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2010,3,15]]}},"alternative-id":["10.1128\/JVI.01616-09"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01616-09","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,3,15]]},"assertion":[{"value":"2009-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-12-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-03-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}