{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T05:25:55Z","timestamp":1791350755476,"version":"4.3.1"},"reference-count":70,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Neutralizing antibodies (NAb) are important for interfering with horizontal transmission of human cytomegalovirus (HCMV) leading to primary and congenital HCMV infection. Recent findings have shown that a pentameric virion complex formed by the glycoproteins gH\/gL, UL128, UL130, and UL131A (UL128C) is required for HCMV entry into epithelial\/endothelial cells (Epi\/EC) and is the target of potent NAb in HCMV-seropositive individuals. Using bacterial artificial chromosome technology, we have generated a modified vaccinia Ankara virus (MVA) that stably coexpresses all 5 rhesus CMV (RhCMV) proteins homologous to HCMV UL128C, termed MVA-RhUL128C. Coimmunoprecipitation confirmed the interaction of RhgH with the other 4 RhCMV subunits of the pentameric complex. All 8 RhCMV-na\u00efve rhesus macaques (RM) vaccinated with MVA-RhUL128C developed NAb that blocked infection of monkey kidney epithelial cells (MKE) and rhesus fibroblasts. NAb titers induced by MVA-RhUL128C measured on both cell types at 2 to 6 weeks postvaccination were comparable to levels observed in naturally infected RM. In contrast, MVA expressing a subset of RhUL128C proteins or RhgB glycoprotein only minimally stimulated NAb that inhibited infection of MKE. In addition, following subcutaneous RhCMV challenge at 8 weeks postvaccination, animals vaccinated with MVA-RhUL128C showed reduced plasma viral loads. These results indicate that MVA expressing the RhUL128C induces NAb inhibiting RhCMV entry into both Epi\/EC and fibroblasts and limits RhCMV replication in RM. This novel approach is the first step in developing a prophylactic HCMV vaccine designed to interfere with virus entry into major cell types permissive for viral replication, a required property of an effective vaccine.<\/jats:p>","DOI":"10.1128\/jvi.01669-12","type":"journal-article","created":{"date-parts":[[2012,11,15]],"date-time":"2012-11-15T03:38:40Z","timestamp":1352950720000},"page":"1322-1332","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":87,"title":["A Vaccine Based on the Rhesus Cytomegalovirus UL128 Complex Induces Broadly Neutralizing Antibodies in Rhesus Macaques"],"prefix":"10.1128","volume":"87","author":[{"given":"Felix","family":"Wussow","sequence":"first","affiliation":[{"name":"Division of Translational Vaccine Research, Beckman Research Institute of the City of Hope, Duarte, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujuan","family":"Yue","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy","family":"Martinez","sequence":"additional","affiliation":[{"name":"Division of Translational Vaccine Research, Beckman Research Institute of the City of Hope, Duarte, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jesse D.","family":"Deere","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeff","family":"Longmate","sequence":"additional","affiliation":[{"name":"California National Primate Research Center, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Cancer Immunotherapeutics and Tumor Immunology, Beckman Research Institute of the City of Hope, Duarte, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter A.","family":"Barry","sequence":"additional","affiliation":[{"name":"Center for Comparative Medicine, University of California, Davis, Davis, California, USA"},{"name":"California National Primate Research Center, University of California, Davis, Davis, California, USA"},{"name":"Department of Pathology and Laboratory Medicine, University of California, Davis, Davis, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Don J.","family":"Diamond","sequence":"additional","affiliation":[{"name":"Division of Translational Vaccine Research, Beckman Research Institute of the City of Hope, Duarte, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/421999"},{"key":"e_1_3_3_3_2","volume-title":"Vaccines for the 21st century: a tool for decisionmaking.","author":"Stratton KR","year":"2001","unstructured":"StrattonKR DurchJS LawrenceRS. 2001. Vaccines for the 21st century: a tool for decisionmaking. National Academy Press, Bethesda, MD."},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2005.09.020"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2004.06.027"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/315022"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0804749"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/171.1.26"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa043337"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199803000-00006"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.64.3.1079-1085.1990"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890430115"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/164.5.835"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.9.4591-4600.2006"},{"key":"e_1_3_3_15_2","first-page":"63","article-title":"Cytomegalovirus cell tropism","volume":"325","author":"Sinzger C","year":"2008","unstructured":"SinzgerC DigelM JahnG. 2008. Cytomegalovirus cell tropism. Curr. Top. Microbiol. Immunol. 325:63\u201383.","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2012.01.001"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.18.10023-10033.2004"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01251-08"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02256-09"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01910-07"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01623-08"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0509201102"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02249-09"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2136652100"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.16.10330-10338.2005"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2011.06.018"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01809-09"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.07.092"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.83523-0"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.12.6620-6636.2003"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0811063106"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.10.040"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.8.4179-4182.2006"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0001638"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.11.056"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2006.09.067"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-008-0074-5"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02631-10"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00883-10"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.2144\/000112096"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-652-8_30"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.192420599"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.1966.tb00871.x"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.8.3965-3976.2004"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.18808-0"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.15.7661-7671.2002"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2008.07.103"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.04.015"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00687-09"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(01)00116-5"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90083-1"},{"key":"e_1_3_3_52_2","article-title":"Generation of recombinant vaccinia viruses","author":"Earl PL","year":"2001","unstructured":"EarlPL MossB WyattLS CarrollMW. 2001. Generation of recombinant vaccinia viruses. Curr. Protoc. Mol. Biol. Chapter 16:Unit 16.","journal-title":"Curr. Protoc. Mol. Biol."},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1002\/rmv.645"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1517\/13543770903584882"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.81921-0"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.01.079"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0804365105"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00467-12"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-7-1537"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.67.1.2"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.11.9576-9583.1999"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200105033441804"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1086\/600882"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1086\/649903"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198204223061601"},{"key":"e_1_3_3_66_2","article-title":"Restoration of viral epithelial tropism improves immunogenicity in rabbits and rhesus macaques for a whole virion vaccine of human cytomegalovirus","author":"Fu TM","unstructured":"FuTM WangD FreedDC TangA LiF HeX ColeS DubeyS FinnefrockAC TerMJ ShiverJW CasimiroDR. Restoration of viral epithelial tropism improves immunogenicity in rabbits and rhesus macaques for a whole virion vaccine of human cytomegalovirus. Vaccine, in press.","journal-title":"Vaccine"},{"key":"e_1_3_3_67_2","article-title":"Antibodies against neutralization epitopes of human cytomegalovirus gH\/gL\/pUL128-130-131 complex and virus spreading may correlate with virus control in vivo","author":"Lilleri D","unstructured":"LilleriD KabanovaA LanzavecchiaA GernaG. Antibodies against neutralization epitopes of human cytomegalovirus gH\/gL\/pUL128-130-131 complex and virus spreading may correlate with virus control in vivo. J. Clin. Immunol., in press.","journal-title":"J. Clin. Immunol."},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.09.056"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.09.135"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60136-0"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(11)70344-9"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01669-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01669-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:38:43Z","timestamp":1646494723000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01669-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2]]},"references-count":70,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1128\/JVI.01669-12"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01669-12","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2]]},"assertion":[{"value":"2012-06-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-01-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}