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However, the longer life cycle of human cytomegalovirus (HCMV) may require more specific interactions with the DNA repair machinery to maximize viral replication. A prototypical damage response to the double-stranded ends of the incoming linear viral DNA was not observed in fibroblasts at early times postinfection (p.i.). Apparently, a constant low level of phosphorylated ATM was enough to phosphorylate its downstream targets, p53 and Nbs1. p53 was the only cellular protein observed to relocate at early times, forming foci in infected cell nuclei between 3.5 and 5.5 h p.i. Approximately half of these foci localized with input viral DNA, and all localized with viral UL112\/113 prereplication site foci. No other DNA repair proteins localized with the virus or prereplication foci in the first 24 h p.i. When viral replication began in earnest, between 24 and 48 h p.i., there were large increases in steady-state levels and phosphorylation of many proteins involved in the damage response, presumably triggered by ATM-Rad3-related kinase activation. However, a sieving process occurred in which only certain proteins were specifically sequestered into viral replication centers and others were particularly excluded. In contrast to other viruses, activation of a damage response is neither necessary nor detrimental to infection, as neither ATM nor Mre11 was required for full virus replication and production. Thus, by preventing simultaneous relocalization of all the necessary repair components to the replication centers, HCMV subverts full activation and completion of both double-stranded break and S-phase checkpoints that should arrest all replication within the cell and likely lead to apoptosis.<\/jats:p>","DOI":"10.1128\/jvi.01670-06","type":"journal-article","created":{"date-parts":[[2006,12,7]],"date-time":"2006-12-07T09:57:14Z","timestamp":1165485434000},"page":"1934-1950","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":116,"title":["Human Cytomegalovirus Disrupts both Ataxia Telangiectasia Mutated Protein (ATM)- and ATM-Rad3-Related Kinase-Mediated DNA Damage Responses during Lytic Infection"],"prefix":"10.1128","volume":"81","author":[{"given":"Min Hua","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Microbiology, Molecular Biology and Biochemistry, and Center for Reproductive Biology, University of Idaho, Moscow, Idaho 83844-3052"},{"name":"State Key Laboratory of Virology, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyle","family":"Rosenke","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Biology and Biochemistry, and Center for Reproductive Biology, University of Idaho, Moscow, Idaho 83844-3052"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila","family":"Czornak","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Biology and Biochemistry, and Center for Reproductive Biology, University of Idaho, Moscow, Idaho 83844-3052"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Fortunato","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Biology and Biochemistry, and Center for Reproductive Biology, University of Idaho, Moscow, Idaho 83844-3052"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8467"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.5.1719-1729.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01368"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200503043"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1159\/000150264"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.104.1.55"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1996.0516"},{"key":"e_1_3_2_9_2","first-page":"2493","year":"1996","unstructured":"Britt, W., and C. 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