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The prevailing model posits that, upon interaction with the target cell, the activating signal is transmitted from the receptor-binding to the fusion-mediating component in a signaling cascade that involves sequential interactions. However, herpesvirus entry proteins may form complexes throughout fusion. Here, we propose that\u2014by analogy with certain eukaryotic signaling cascades\u2014transmission of the activating signal involves pre-assembled complexes and allosteric effects.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.01704-24","type":"journal-article","created":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T14:00:51Z","timestamp":1767621651000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":1,"title":["Pre-assembled complexes and allosteric effects: parallels between eukaryotic phosphorylation cascades and membrane fusion during herpesviral entry"],"prefix":"10.1128","volume":"100","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0365-8583","authenticated-orcid":false,"given":"Gonzalo L.","family":"Gonzalez-Del Pino","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05wvpxv85","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Microbiology and Tufts NIH-IRACDA Program, Tufts University School of Medicine","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3113-6958","authenticated-orcid":true,"given":"Ekaterina E.","family":"Heldwein","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05wvpxv85","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular Biology and Microbiology and Tufts NIH-IRACDA Program, Tufts University School of Medicine","place":["Boston, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.03.043"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-111821-093413"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/v4050800"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.21775\/cimb.041.063"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/v14020296"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00448-w"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01336-18"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2012.10.005"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/v7122957"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401883101"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.005728"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600875"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01906-14"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/s1097-2765(01)00298-2"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002277"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1004309"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02625-21"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.1837"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1011806108"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-19617-9"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abm2546"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.aan1457"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1111333108"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","unstructured":"Gonz\u00e1lez-Del Pino GL Walsh RM Atanasiu D Cairns TM Saw WT Cohen GH Heldwein EE. 2024. 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