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Most protein interactions are not binary or easily amenable to structural determination. Using infectious preparations of a polerovirus (\n            <jats:named-content content-type=\"genus-species\">Potato leafroll virus<\/jats:named-content>\n            [PLRV]) and protein interaction reporter (PIR), a revolutionary technology that couples a mass spectrometric-cleavable chemical cross-linker with high-resolution mass spectrometry, we provide the first report of a host-pathogen protein interaction network that includes data-derived, topological features for every cross-linked site that was identified. We show that PLRV virions have hot spots of protein interaction and multifunctional surface topologies, revealing how these plant viruses maximize their use of binding interfaces. Modeling data, guided by cross-linking constraints, suggest asymmetric packing of the major capsid protein in the virion, which supports previous epitope mapping studies. Protein interaction topologies are conserved with other species in the\n            <jats:named-content content-type=\"genus-species\">Luteoviridae<\/jats:named-content>\n            and with unrelated viruses in the\n            <jats:named-content content-type=\"genus-species\">Herpesviridae<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Adenoviridae<\/jats:named-content>\n            . Functional analysis of three PLRV-interacting host proteins\n            <jats:italic>in planta<\/jats:italic>\n            using a reverse-genetics approach revealed a complex, molecular tug-of-war between host and virus. Structural mimicry and diversifying selection\u2014hallmarks of host-pathogen interactions\u2014were identified within host and viral binding interfaces predicted by our models. These results illuminate the functional diversity of the PLRV-host protein interaction network and demonstrate the usefulness of PIR technology for precision mapping of functional host-pathogen protein interaction topologies.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            The exterior shape of a plant virus and its interacting host and insect vector proteins determine whether a virus will be transmitted by an insect or infect a specific host. Gaining this information is difficult and requires years of experimentation. We used protein interaction reporter (PIR) technology to illustrate how viruses exploit host proteins during plant infection. PIR technology enabled our team to precisely describe the sites of functional virus-virus, virus-host, and host-host protein interactions using a mass spectrometry analysis that takes just a few hours. Applications of PIR technology in host-pathogen interactions will enable researchers studying recalcitrant pathogens, such as animal pathogens where host proteins are incorporated directly into the infectious agents, to investigate how proteins interact during infection and transmission as well as develop new tools for interdiction and therapy.\n          <\/jats:p>","DOI":"10.1128\/jvi.01706-15","type":"journal-article","created":{"date-parts":[[2015,12,11]],"date-time":"2015-12-11T12:54:04Z","timestamp":1449838444000},"page":"1973-1987","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Visualization of Host-Polerovirus Interaction Topologies Using Protein Interaction Reporter Technology"],"prefix":"10.1128","volume":"90","author":[{"given":"Stacy L.","family":"DeBlasio","sequence":"first","affiliation":[{"name":"Boyce Thompson Institute for Plant Research, Ithaca, New York, USA"},{"name":"USDA-Agricultural Research Service, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan D.","family":"Chavez","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariko M.","family":"Alexander","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute for Plant Research, Ithaca, New York, USA"},{"name":"Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Ramsey","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute for Plant Research, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimmy K.","family":"Eng","sequence":"additional","affiliation":[{"name":"University of Washington Proteomics Resources, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaclyn","family":"Mahoney","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute for Plant Research, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stewart M.","family":"Gray","sequence":"additional","affiliation":[{"name":"USDA-Agricultural Research Service, Ithaca, New York, USA"},{"name":"Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James E.","family":"Bruce","sequence":"additional","affiliation":[{"name":"Department of Genome Sciences, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Cilia","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute for Plant Research, Ithaca, New York, USA"},{"name":"USDA-Agricultural Research Service, Ithaca, New York, USA"},{"name":"Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"271","volume-title":"Phloem: molecular cell biology, systemic communication, and biotic interactions","author":"Stewart LR","year":"2013","unstructured":"Stewart LR, Ding B, Falk BW. 2013. 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