{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,26]],"date-time":"2025-12-26T03:29:10Z","timestamp":1766719750833},"reference-count":25,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Alternative splicing of the precursor mRNA (pre-mRNA) of human parvovirus B19 (B19V) plays a key role in posttranscriptional regulation of B19V gene expression. We report that the central exon of the B19V pre-mRNA is defined by three GAA motif-containing exonic splicing enhancers and a G\/GU-rich intronic splicing enhancer that lies adjacent to the second donor site. Moreover, targeting of morpholino antisense oligonucleotides to the two splicing enhancers surrounding the second donor site led to a significant reduction in splicing at this donor site during B19V infection of permissive CD36\n            <jats:sup>+<\/jats:sup>\n            erythroid progenitor cells.\n          <\/jats:p>","DOI":"10.1128\/jvi.01708-10","type":"journal-article","created":{"date-parts":[[2010,12,16]],"date-time":"2010-12-16T04:04:41Z","timestamp":1292472281000},"page":"2463-2468","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Inclusion of the Central Exon of Parvovirus B19 Precursor mRNA Is Determined by Multiple Splicing Enhancers in both the Exon and the Downstream Intron"],"prefix":"10.1128","volume":"85","author":[{"given":"Wuxiang","family":"Guan","sequence":"first","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology; University of Kansas Medical Center, Kansas City, Kansas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology; University of Kansas Medical Center, Kansas City, Kansas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinfeng","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology; University of Kansas Medical Center, Kansas City, Kansas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steve","family":"Kleiboeker","sequence":"additional","affiliation":[{"name":"ViraCor Laboratories, Lee's Summit, Missouri"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianming","family":"Qiu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Genetics and Immunology; University of Kansas Medical Center, Kansas City, Kansas"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg616"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-04-215756"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.2.1309-1319.1999"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf668"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S1355838200000960"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01162-08"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00702-09"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.10.13.1569"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.04.172"},{"key":"e_1_3_2_11_2","first-page":"Unit","volume":"2008","year":"2008","unstructured":"Moulton, J. D., and Y. L. Yan. 2008. Using morpholinos to control gene expression. Curr. Protoc. Mol. Biol. 2008:Unit 26.8.","journal-title":"Curr. Protoc. Mol. Biol."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.6.6.1107"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.61.8.2395-2406.1987"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.287"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.2.654-662.2006"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00714-07"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.24.12435-12447.2002"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312734200"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90466-O"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddl171"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90126-V"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02247-07"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.3.1604-1609.2006"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra030840"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2003.09.011"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90064-4"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01708-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.01708-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T16:25:32Z","timestamp":1646497532000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.01708-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,3]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2011,3]]}},"alternative-id":["10.1128\/JVI.01708-10"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01708-10","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,3]]},"assertion":[{"value":"2010-08-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-12-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-02-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}