{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T21:09:52Z","timestamp":1791234592708,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,23]],"date-time":"2025-12-23T00:00:00Z","timestamp":1766448000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Guangdong Basic and Applied Basic Research Foundation","doi-asserted-by":"publisher","award":["2022A1515011156"],"award-info":[{"award-number":["2022A1515011156"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["no. 82071352"],"award-info":[{"award-number":["no. 82071352"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["no. 82101775"],"award-info":[{"award-number":["no. 82101775"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["no. 82171675"],"award-info":[{"award-number":["no. 82171675"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["no. 82341094"],"award-info":[{"award-number":["no. 82341094"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Marine Economy Development Special Project","award":["NO. GDNRC [2022]35"],"award-info":[{"award-number":["NO. GDNRC [2022]35"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2025,12,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Infection by the Zika virus (ZIKV) during pregnancy can cause congenital Zika syndrome (CZS) or other central nervous system conditions in infants, underscoring the importance of understanding the role of ZIKV-induced placental damage in the development of CZS. Here, we established a ZIKV-infected pregnant mouse model and examined the pathological changes in the placenta following ZIKV infection. We found that ZIKV infection induces severe placental inflammation associated with trophoblast PANoptosis. Specifically, ZIKV activates RIG-I and recruits ASC, caspase-1, NLRP3, caspase-8, and RIPK1 to form a PANoptosome complex, leading to the activation of PANoptosis. Moreover, the combined use of Z-VAD-FMK with GSK872 or with the RIG-I inhibitor RIG012 robustly suppressed ZIKV-induced cell death, attenuated the inflammatory response in trophoblast cells and the placenta induced by ZIKV infection. Collectively, we elucidate a previously unrecognized mechanism by which ZIKV infection causes severe placental inflammation by activating PANoptosis and provide a foundation for the potential application of anti-PANoptotic therapy against ZIKV-associated diseases.<\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Zika virus (ZIKV), a mosquito-borne virus, has caused significant disease in humans during outbreaks over the last decade. Currently, there is no approved preventive vaccine or specific therapeutic drug against ZIKV. The World Health Organization declared a Public Health Emergency of International Concern regarding microcephaly and other neurological disorders caused by ZIKV during pregnancy in 2016, highlighting the importance of understanding the role of the maternal-fetal barrier in this viral disease. The mechanism by which ZIKV causes placental pathogenesis, however, remains unclear. In this study, our data elucidate a previously unrecognized mechanism underlying ZIKV infection that causes severe placental inflammation by activating PANoptosis. Furthermore, we propose a treatment that effectively inhibits ZIKV-induced PANoptosis and attenuates the inflammatory response in trophoblast cells\n                        <jats:italic toggle=\"yes\">in vitro<\/jats:italic>\n                        and\n                        <jats:italic toggle=\"yes\">in vivo<\/jats:italic>\n                        .\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.01759-25","type":"journal-article","created":{"date-parts":[[2025,11,20]],"date-time":"2025-11-20T14:00:17Z","timestamp":1763647217000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["ZIKV infection causes placental inflammation through activating PANoptosis"],"prefix":"10.1128","volume":"99","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0707-4438","authenticated-orcid":false,"given":"Shuqing","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences, Southern Medical University","place":["Guangzhou, China"]},{"name":"Clinical Experimental Center, Jiangmen Engineering Technology Research Center of Clinical Biobank and Translational Research, Jiangmen Key Laboratory of Precision and Clinical Translation Medicine, Jiangmen Central Hospital","place":["Jiangmen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kexin","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Public Health, Sun Yat-sen University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minjie","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Public Health, Sun Yat-sen University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Southern Medical University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linyue","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyao","family":"Liang","sequence":"additional","affiliation":[{"name":"School of Public Health, Sun Yat-sen University","place":["Guangzhou, 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China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangwei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Public Health, Sun Yat-sen University","place":["Guangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingwen","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Southern Medical University","place":["Guangzhou, China"]},{"name":"Clinical Experimental Center, Jiangmen Engineering Technology Research Center of Clinical Biobank and Translational Research, Jiangmen Key Laboratory of Precision and Clinical Translation Medicine, Jiangmen Central Hospital","place":["Jiangmen, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xun","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Zhongshan School of Medicine, Sun Yat-sen University","place":["Guangzhou, China"]},{"name":"Key Laboratory of Tropical Disease 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