{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:09:01Z","timestamp":1791248941105,"version":"4.1.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    JC virus (JCV)-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    cytotoxic T lymphocytes (CTL) are associated with a favorable outcome in patients with progressive multifocal leukoencephalopathy (PML) and cross-recognize the polyomavirus BK virus (BKV). We sought to determine the frequency and phenotype in fresh blood of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells specific for two A*0201-restricted JCV epitopes, VP1\n                    <jats:sub>p36<\/jats:sub>\n                    and VP1\n                    <jats:sub>p100<\/jats:sub>\n                    , and assess their impact on JC and BK viremia and viruria in 15 healthy subjects, eight human immunodeficiency virus-positive (HIV\n                    <jats:sup>+<\/jats:sup>\n                    ) individuals, and nine HIV\n                    <jats:sup>+<\/jats:sup>\n                    patients with PML (HIV\n                    <jats:sup>+<\/jats:sup>\n                    PML patients) classified as survivors. After magnetic preenrichment of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, epitope-specific cells ranged from 0.001% to 0.22% by tetramer staining, with no significant difference among the three study groups. By use of seven-color flow cytometry, there was no predominant differentiation phenotype subset among JCV-specific CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells in healthy individuals, HIV\n                    <jats:sup>+<\/jats:sup>\n                    subjects, or HIV\n                    <jats:sup>+<\/jats:sup>\n                    PML patients. However, in one HIV\n                    <jats:sup>+<\/jats:sup>\n                    PML patient studied in the acute phase, there was a majority of activated effector memory cells. BKV DNA was undetectable in all blood samples by quantitative PCR, while a low JC viral load was found in the blood of only one HIV\n                    <jats:sup>+<\/jats:sup>\n                    and two HIV\n                    <jats:sup>+<\/jats:sup>\n                    PML patients. JCV and BKV DNA were detected in 33.3% and 13.3% of all urine samples, respectively, independent of the presence of JCV-specific CTL. The detection of JCV DNA in the urine was associated with the presence of a JCV VP1\n                    <jats:sub>p100<\/jats:sub>\n                    CTL response. Immunotherapies aiming at increasing the cellular immune response against JCV may be valuable in the treatment of HIV\n                    <jats:sup>+<\/jats:sup>\n                    individuals with PML.\n                  <\/jats:p>","DOI":"10.1128\/jvi.01809-06","type":"journal-article","created":{"date-parts":[[2007,1,17]],"date-time":"2007-01-17T20:34:42Z","timestamp":1169066082000},"page":"3361-3368","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Frequency and Phenotype of JC Virus-Specific CD8\n                    <sup>+<\/sup>\n                    T Lymphocytes in the Peripheral Blood of Patients with Progressive Multifocal Leukoencephalopathy"],"prefix":"10.1128","volume":"81","author":[{"given":"Marco A.","family":"Lima","sequence":"first","affiliation":[{"name":"Department of Neurology"},{"name":"Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts"},{"name":"School of Medicine, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Marzocchetti","sequence":"additional","affiliation":[{"name":"Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Autissier","sequence":"additional","affiliation":[{"name":"Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Troy","family":"Tompkins","sequence":"additional","affiliation":[{"name":"Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiping","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Gordon","sequence":"additional","affiliation":[{"name":"Center for Neurovirology and Cancer Biology, Temple University, Philadelphia, Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David B.","family":"Clifford","sequence":"additional","affiliation":[{"name":"Departments of Neurology and Medicine, Washington University School of Medicine, St. Louis, Missouri"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajesh T.","family":"Gandhi","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nagagopal","family":"Venna","sequence":"additional","affiliation":[{"name":"Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph R.","family":"Berger","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Kentucky College of Medicine, Lexington, Kentucky"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Igor J.","family":"Koralnik","sequence":"additional","affiliation":[{"name":"Department of Neurology"},{"name":"Division of Viral Pathogenesis, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/13550280152537184"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0402-379"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe058122"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/35065118"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.7.3495-3505.2006"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/13550280390195298"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.aids.0000194804.97164.86"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.22.11918-11926.2003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/awh215"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.18.10206-10210.2004"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103492"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.20492"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-11-3577"},{"key":"e_1_3_2_15_2","doi-asserted-by":"crossref","first-page":"1780","DOI":"10.4049\/jimmunol.162.3.1780","volume":"162","year":"1999","unstructured":"Gray, C. 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