{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T22:35:17Z","timestamp":1768343717717,"version":"3.49.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2019,3,15]],"date-time":"2019-03-15T00:00:00Z","timestamp":1552608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2016M3A9B6918676"],"award-info":[{"award-number":["2016M3A9B6918676"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018R1A2B2005086"],"award-info":[{"award-number":["2018R1A2B2005086"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2018R1A1A3A04077961"],"award-info":[{"award-number":["2018R1A1A3A04077961"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2019,3,15]]},"abstract":"<jats:p>\n            With the widespread emergence of NAI-resistant influenza virus strains, continuous monitoring of mutations that confer antiviral resistance is needed. Laninamivir is the most recently approved NAI in several countries; few data exist related to the\n            <jats:italic>in vitro<\/jats:italic>\n            selection of viral mutations conferring resistance to laninamivir. Thus, we screened and identified substitutions conferring resistance to laninamivir by random mutagenesis of the NA gene of the 2009 pandemic influenza [A(H1N1)pdm09] virus strain followed by deep sequencing of the laninamivir-selected variants. We found several novel substitutions in NA (D199E and P458T) in an A(H1N1)pdm09 background which conferred resistance to NAIs and which had an impact on viral fitness. Our study highlights the importance of continued surveillance for potential antiviral-resistant variants and the development of alternative therapeutics.\n          <\/jats:p>","DOI":"10.1128\/jvi.01825-18","type":"journal-article","created":{"date-parts":[[2019,1,3]],"date-time":"2019-01-03T13:17:55Z","timestamp":1546521475000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["<i>In Vitro<\/i>\n            and\n            <i>In Vivo<\/i>\n            Characterization of Novel Neuraminidase Substitutions in Influenza A(H1N1)pdm09 Virus Identified Using Laninamivir-Mediated\n            <i>In Vitro<\/i>\n            Selection"],"prefix":"10.1128","volume":"93","author":[{"given":"Khristine Kaith S.","family":"Lloren","sequence":"first","affiliation":[{"name":"College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea"},{"name":"College of Veterinary Medicine and Agricultural Sciences, De La Salle Araneta University, Potrero Malabon City, Philippines"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4500-1280","authenticated-orcid":false,"given":"Jin Jung","family":"Kwon","sequence":"additional","affiliation":[{"name":"College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5120-0374","authenticated-orcid":false,"given":"Won-Suk","family":"Choi","sequence":"additional","affiliation":[{"name":"College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ju Hwan","family":"Jeong","sequence":"additional","affiliation":[{"name":"College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Su Jeong","family":"Ahn","sequence":"additional","affiliation":[{"name":"College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Young Ki","family":"Choi","sequence":"additional","affiliation":[{"name":"College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun Hee","family":"Baek","sequence":"additional","affiliation":[{"name":"College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6073-0783","authenticated-orcid":false,"given":"Min-Suk","family":"Song","sequence":"additional","affiliation":[{"name":"College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2011.01.009"},{"key":"e_1_3_2_3_2","unstructured":"World Health Organization. 2009. 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