{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2022,3,29]],"date-time":"2022-03-29T18:24:47Z","timestamp":1648578287823},"reference-count":32,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2013,11,1]],"date-time":"2013-11-01T00:00:00Z","timestamp":1383264000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Based on a DNA sequence and relative genomic position similar to those other herpesviruses, varicella-zoster virus (VZV) open reading frame 48 (ORF48) is predicted to encode an alkaline nuclease. Here we report the cloning, expression, purification, and characterization of recombinant VZV ORF48 protein and a VZV ORF48 point mutation (T172P). Protein encoded by wild-type ORF48, but not mutant protein, displayed both endo- and exonuclease activity, identifying ORF48 as a potential therapeutic target in VZV disease since efficient viral replication requires viral nuclease activity.<\/jats:p>","DOI":"10.1128\/jvi.01879-13","type":"journal-article","created":{"date-parts":[[2013,8,22]],"date-time":"2013-08-22T04:00:07Z","timestamp":1377144007000},"page":"11936-11938","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":2,"title":["Varicella-Zoster Virus Open Reading Frame 48 Encodes an Active Nuclease"],"prefix":"10.1128","volume":"87","author":[{"given":"Niklaus H.","family":"Mueller","sequence":"first","affiliation":[{"name":"Departments of Ophthalmology, University of Colorado, School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Don","family":"Gilden","sequence":"additional","affiliation":[{"name":"Neurology, University of Colorado, School of Medicine, Aurora, Colorado, USA"},{"name":"Microbiology, University of Colorado, School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Randall J.","family":"Cohrs","sequence":"additional","affiliation":[{"name":"Neurology, University of Colorado, School of Medicine, Aurora, Colorado, USA"},{"name":"Microbiology, University of Colorado, School of Medicine, Aurora, Colorado, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/306478a0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03210846"},{"key":"e_1_3_2_4_2","first-page":"2773","volume-title":"Fields virology","author":"Cohen JI","year":"2007","unstructured":"CohenJIStrausSEArvinAM. 2007. 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