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Gaining a better understanding of why intact proviruses decay faster\n            <jats:italic>in vitro<\/jats:italic>\n            might help the field identify strategies to purge the reservoir\n            <jats:italic>in vivo<\/jats:italic>\n            . When used wisely,\n            <jats:italic>in vitro<\/jats:italic>\n            models are a powerful tool to study the selective pressures shaping the viral landscape. Our finding that massively deleted sequences rarely succeed in integrating has several ramifications. It demonstrates that the total HIV DNA can differ substantially in character from the integrated HIV DNA under certain circumstances. The presence of unintegrated HIV DNA has the potential to obscure selection pressures and confound the interpretation of clinical studies, especially in the case of trials involving treatment interruptions.\n          <\/jats:p>","DOI":"10.1128\/jvi.01900-19","type":"journal-article","created":{"date-parts":[[2020,2,12]],"date-time":"2020-02-12T13:33:00Z","timestamp":1581514380000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Next-Generation Sequencing in a Direct Model of HIV Infection Reveals Important Parallels to and Differences from\n            <i>In Vivo<\/i>\n            Reservoir Dynamics"],"prefix":"10.1128","volume":"94","author":[{"given":"Marilia Rita","family":"Pinzone","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Paola","family":"Bertuccio","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA"},{"name":"Department of Biomedical and Dental Sciences and Morphofunctional Imaging, University of Messina, Messina, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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