{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:30:02Z","timestamp":1787016602277,"version":"build-2736575974"},"reference-count":108,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T00:00:00Z","timestamp":1710806400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T00:00:00Z","timestamp":1710806400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["410921131"],"award-info":[{"award-number":["410921131"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["390874280"],"award-info":[{"award-number":["390874280"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100009139","name":"Deutsches Zentrum f\u00fcr Infektionsforschung","doi-asserted-by":"crossref","award":["TTU 0.5.910"],"award-info":[{"award-number":["TTU 0.5.910"]}],"id":[{"id":"10.13039\/100009139","id-type":"DOI","asserted-by":"crossref"}]},{"name":"National Insitute for Allergy and Infectious Disease","award":["R01AI107301"],"award-info":[{"award-number":["R01AI107301"]}]},{"name":"Hannover Biomedical Research School"},{"name":"Center for Infection Biology"},{"DOI":"10.13039\/501100003107","name":"Bundesministerium f\u00fcr Gesundheit","doi-asserted-by":"crossref","award":["1-2516-FSB-416"],"award-info":[{"award-number":["1-2516-FSB-416"]}],"id":[{"id":"10.13039\/501100003107","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Nationale Forschungsplatform f\u00fcr Zoonosen","award":["VIRASCREEN"],"award-info":[{"award-number":["VIRASCREEN"]}]},{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"crossref","award":["VirBio 01KI2106"],"award-info":[{"award-number":["VirBio 01KI2106"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2024,3,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>Hepatitis C virus (HCV) infection progresses to chronicity in the majority of infected individuals. Its high intra-host genetic variability enables HCV to evade the continuous selection pressure exerted by the host, contributing to persistent infection. Utilizing a cell culture-adapted HCV population (p100pop) which exhibits increased replicative capacity in various liver cell lines, this study investigated virus and host determinants that underlie enhanced viral fitness. Characterization of a panel of molecular p100 clones revealed that cell culture adaptive mutations optimize a range of virus-host interactions, resulting in expanded cell tropism, altered dependence on the cellular co-factor micro-RNA 122 and increased rates of virus spread. On the host side, comparative transcriptional profiling of hepatoma cells infected either with p100pop or its progenitor virus revealed that enhanced replicative fitness correlated with activation of endoplasmic reticulum stress signaling and the unfolded protein response. In contrast, infection of primary human hepatocytes with p100pop led to a mild attenuation of virion production which correlated with a greater induction of cell-intrinsic antiviral defense responses. In summary, long-term passage experiments in cells where selective pressure from innate immunity is lacking improves multiple virus-host interactions, enhancing HCV replicative fitness. However, this study further indicates that HCV has evolved to replicate at low levels in primary human hepatocytes to minimize innate immune activation, highlighting that an optimal balance between replicative fitness and innate immune induction is key to establish persistence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>Hepatitis C virus (HCV) infection remains a global health burden with 58 million people currently chronically infected. However, the detailed molecular mechanisms that underly persistence are incompletely defined. We utilized a long-term cell culture-adapted HCV, exhibiting enhanced replicative fitness in different human liver cell lines, in order to identify molecular principles by which HCV optimizes its replication fitness. Our experimental data revealed that cell culture adaptive mutations confer changes in the host response and usage of various host factors. The latter allows functional flexibility at different stages of the viral replication cycle. However, increased replicative fitness resulted in an increased activation of the innate immune system, which likely poses boundary for functional variation in authentic hepatocytes, explaining the observed attenuation of the adapted virus population in primary hepatocytes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/jvi.01921-23","type":"journal-article","created":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T09:00:18Z","timestamp":1707210018000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":5,"title":["Unraveling the dynamics of hepatitis C virus adaptive mutations and their impact on antiviral responses in primary human hepatocytes"],"prefix":"10.1128","volume":"98","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-6493-8187","authenticated-orcid":false,"given":"Nicola","family":"Frericks","sequence":"first","affiliation":[{"name":"Institute for Experimental Virology, TWINCORE, Hannover, Germany"},{"name":"Department for Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard J. P.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department for Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany"},{"name":"Division of Veterinary Medicine, Paul Ehrlich Institute, Langen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birthe M.","family":"Reinecke","sequence":"additional","affiliation":[{"name":"Institute for Experimental Virology, TWINCORE, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maike","family":"Herrmann","sequence":"additional","affiliation":[{"name":"Division of Veterinary Medicine, Paul Ehrlich Institute, Langen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yannick","family":"Br\u00fcggemann","sequence":"additional","affiliation":[{"name":"Department for Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Todt","sequence":"additional","affiliation":[{"name":"Department for Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany"},{"name":"European Virus Bioinformatics Center (EVBC), Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Csaba","family":"Miskey","sequence":"additional","affiliation":[{"name":"Division of Medical Biotechnology, Paul Ehrlich Institute, Langen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florian W. R.","family":"Vondran","sequence":"additional","affiliation":[{"name":"Department for General, Visceral and Transplant Surgery, Hannover Medical School, Hannover, Germany"},{"name":"Clinic for General, Visceral and Transplant Surgery, University Hospital RWTH Aachen, Aachen, Germany"},{"name":"German Center for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eike","family":"Steinmann","sequence":"additional","affiliation":[{"name":"Department for Molecular and Medical Virology, Ruhr-University Bochum, Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6789-4422","authenticated-orcid":false,"given":"Thomas","family":"Pietschmann","sequence":"additional","affiliation":[{"name":"Institute for Experimental Virology, TWINCORE, Hannover, Germany"},{"name":"German Center for Infection Research (DZIF), Partner Site Hannover-Braunschweig, Hannover, Germany"},{"name":"Cluster of Excellence RESIST (EXC 2155), Hannover Medical School, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8240-0010","authenticated-orcid":false,"given":"Julie","family":"Sheldon","sequence":"additional","affiliation":[{"name":"Institute for Experimental Virology, TWINCORE, Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","unstructured":"WHO. 2021. Global progress report on HIV viral hepatitis and sexual transmitted infections."},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.66.5.3225-3229.1992"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003359"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00059-11"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.247"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14899"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2018.11.012"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02824-12"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01860-14"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2019-319354"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.abd3233"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22550"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00891-09"},{"key":"e_1_3_3_15_2","first-page":"1","article-title":"Establishment of a cell line and its Clonal Sublines from a patient with Hepatoblastoma","volume":"67","author":"Doi I","year":"1976","unstructured":"Doi I. 1976. Establishment of a cell line and its Clonal Sublines from a patient with Hepatoblastoma. Gan 67:1\u201310. doi:","journal-title":"Gan"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/282615a0"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01504-09"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26567"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1114016"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05843-11"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky273"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1213515110"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt075"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-05053-3"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky662"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/mSphere.00009-15"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000978"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01102-13"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep45605"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00245-21"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms6408"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.24.12047-12057.2001"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.66.3.1476-1483.1992"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1995.tb00289.x"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkp553"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkz194"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.11.4942"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04220-14"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(16)31715-9"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2103671118"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01019"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-019-0425-6"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1010812"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1057082"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.3390\/v4102251"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2009.11.004"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.07101-11"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02418-10"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.022186-0"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.320085"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2010.05.032"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.4161\/auto.5.7.9243"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0907344106"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.4161\/auto.27954"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.4161\/cc.8.10.8498"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2012.09.004"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000546"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01362-07"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1008271"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(78)90223-4"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.68.7.4295-4301.1994"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v24.i12.1299"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0044965"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.5.3187-3194.2005"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0405152101"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000475"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.212532699"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.76.8.4008-4021.2002"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.282.5390.938"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.00910"},{"key":"e_1_3_3_71_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01548-10"},{"key":"e_1_3_3_72_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22673"},{"key":"e_1_3_3_73_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.32772"},{"key":"e_1_3_3_74_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1631"},{"key":"e_1_3_3_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01185-15"},{"key":"e_1_3_3_76_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002144"},{"key":"e_1_3_3_77_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001278"},{"key":"e_1_3_3_78_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02268-10"},{"key":"e_1_3_3_79_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01890-06"},{"key":"e_1_3_3_80_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000035"},{"key":"e_1_3_3_81_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00826-08"},{"key":"e_1_3_3_82_2","doi-asserted-by":"publisher","DOI":"10.3390\/v2081734"},{"key":"e_1_3_3_83_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02614-07"},{"key":"e_1_3_3_84_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.007625"},{"key":"e_1_3_3_85_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02550-08"},{"key":"e_1_3_3_86_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M809244200"},{"key":"e_1_3_3_87_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00393-11"},{"key":"e_1_3_3_88_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.6b00143"},{"key":"e_1_3_3_89_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.04958-14"},{"key":"e_1_3_3_90_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2014.01.055"},{"key":"e_1_3_3_91_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.52237"},{"key":"e_1_3_3_92_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.9.5208"},{"key":"e_1_3_3_93_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0504877103"},{"key":"e_1_3_3_94_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02824-12"},{"key":"e_1_3_3_95_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5424.110"},{"key":"e_1_3_3_96_2","first-page":"3858","article-title":"Growth of human hepatoma cells lines with differentiated functions in chemically defined medium","volume":"42","author":"Nakabayashi H","year":"1982","unstructured":"Nakabayashi H, Taketa K, Miyano K, Yamane T, Sato J. 1982. Growth of human hepatoma cells lines with differentiated functions in chemically defined medium. Cancer Res 42:3858\u20133863. doi:","journal-title":"Cancer Res"},{"key":"e_1_3_3_97_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.76.24.13001-13014.2002"},{"key":"e_1_3_3_98_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.5.2689-2699.2005"},{"key":"e_1_3_3_99_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0101386"},{"key":"e_1_3_3_100_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24692"},{"key":"e_1_3_3_101_2","unstructured":"Binder M. TCID50_calculator_v2_17-01-20_MB. Available from: https:\/\/www.klinikum.uni-heidelberg.de\/zentrum-fuer-infektiologie\/molecular-virology\/welcome\/downloads"},{"key":"e_1_3_3_102_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"e_1_3_3_103_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2006-7-10-r100"},{"key":"e_1_3_3_104_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2202653119"},{"key":"e_1_3_3_105_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1491"},{"key":"e_1_3_3_106_2","unstructured":"Anonymous. 2022. R: a language and environment for statistical computing. R Foundation for Statistical Computing on R Core Team. Available from: https:\/\/www.R-project.org"},{"key":"e_1_3_3_107_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141"},{"key":"e_1_3_3_108_2","volume-title":"EnhancedVolcano: publication-ready volcano plots with enhanced colouring and labeling. R package version 1.16.0","author":"Blighe K","year":"2022","unstructured":"Blighe K, Rana S, Lewis M. 2022. EnhancedVolcano: publication-ready volcano plots with enhanced colouring and labeling. R package version 1.16.0. https:\/\/github.com\/kevinblighe\/EnhancedVolcano.Accessed."},{"key":"e_1_3_3_109_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-0-387-98141-3"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.01921-23","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/jvi.01921-23","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T14:20:48Z","timestamp":1726496448000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/jvi.01921-23"}},"subtitle":[],"editor":[{"given":"J.-H. James","family":"Ou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2024,3,19]]},"references-count":108,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2024,3,19]]}},"alternative-id":["10.1128\/jvi.01921-23"],"URL":"https:\/\/doi.org\/10.1128\/jvi.01921-23","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,3,19]]},"assertion":[{"value":"2023-12-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-02-06","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01921-23"}}