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The cellular and viral proteins that participate in these processes are expected to interact with the UTRs. In this study, we used biotinylated RNA-protein pulldown and liquid chromatography-mass spectrometry\/mass spectrometry (LC-MS\/MS) analyses to identify that the far upstream element (FUSE) binding protein 1 (FBP1) binds with JEV 5\u2032 and 3\u2032 UTRs. The impact of FBP1 on JEV infection was determined in cells with altered FBP1 expression. JEV replication was enhanced by knockdown and reduced by the overexpression of FBP1, indicating a negative role for FBP1 in JEV infection. FBP1, a nuclear protein, was redistributed to the perinuclear region and appeared as cytoplasmic foci that partially colocalized with JEV RNA in the early stage of JEV infection. By using a JEV replicon reporter assay, FBP1 appeared to suppress JEV protein expression mediated by the 5\u2032 and 3\u2032 UTRs. Thus, we suggest that FBP1 binds with the JEV UTR RNA and functions as a host anti-JEV defense molecule by repressing viral protein expression.<\/jats:p>","DOI":"10.1128\/jvi.01950-10","type":"journal-article","created":{"date-parts":[[2011,3,4]],"date-time":"2011-03-04T09:12:48Z","timestamp":1299229968000},"page":"4698-4706","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["FUSE Binding Protein 1 Interacts with Untranslated Regions of Japanese Encephalitis Virus RNA and Negatively Regulates Viral Replication"],"prefix":"10.1128","volume":"85","author":[{"given":"Hsu-Ling","family":"Chien","sequence":"first","affiliation":[{"name":"Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan"},{"name":"Institute of Biomedical Sciences, Academia Sinica, Taipei, 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