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Dunn Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100006988","id-type":"DOI","asserted-by":"crossref"}]},{"name":"HHS | U.S. Public Health Service","award":["1R21AI166985"],"award-info":[{"award-number":["1R21AI166985"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2024,2,20]]},"abstract":"<jats:p>Guanarito virus (GTOV) is a rodent-borne virus. GTOV causes fever, prostration, headache, arthralgia, cough, sore throat, nausea, vomiting, diarrhea, epistaxis, bleeding gums, menorrhagia, and melena in humans. The lethality rate is 23.1% or higher. Vero cell-adapted GTOV S-26764 shows a clear cytopathic effect (CPE), whereas the parental virus shows unclear CPE in Vero cells. We generated a reverse genetics system to rescue recombinant GTOVs and found that E1497K in the L protein was responsible for the formation of clear plaques as well as enhanced viral RNA replication and transcription efficiency. 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