{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:27:53Z","timestamp":1789662473500,"version":"4.0.1"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>CD8<jats:sup>+<\/jats:sup>T cells (T<jats:sub>CD8<jats:sup>+<\/jats:sup><\/jats:sub>) play a crucial role in immunity to viruses. Antiviral T<jats:sub>CD8<jats:sup>+<\/jats:sup><\/jats:sub>are initially activated by recognition of major histocompatibility complex (MHC) class I-peptide complexes on the surface of professional antigen-presenting cells (pAPC). Migration of pAPC from the site of infection to secondary lymphoid organs is likely required during a natural infection. Migrating pAPC can be directly infected with virus or may internalize antigen derived from virus-infected cells. The use of experimental virus infections to assess the requirement for pAPC migration in initiation of T<jats:sub>CD8<jats:sup>+<\/jats:sup><\/jats:sub>responses has proven difficult to interpret because injected virus can readily drain to secondary lymphoid organs without the need for cell-mediated transport. To overcome this ambiguity, we examined the generation of antigen-specific T<jats:sub>CD8<jats:sup>+<\/jats:sup><\/jats:sub>after immunization with recombinant adenoviruses that express antigen driven by skin-specific or ubiquitous promoters. We show that the induction of T<jats:sub>CD8<jats:sup>+<\/jats:sup><\/jats:sub>in response to tissue-targeted antigen is less efficient than the response to ubiquitously expressed antigen and that the resulting T<jats:sub>CD8<jats:sup>+<\/jats:sup><\/jats:sub>fail to clear all target cells pulsed with the antigenic peptide. This failure to prime a fully functional T<jats:sub>CD8<jats:sup>+<\/jats:sup><\/jats:sub>response results from a reduced period of priming to peripherally expressed antigen versus ubiquitously expressed antigen and correlated with a brief burst of pAPC migration from the skin, a requirement for induction of the response to peripheral antigen. These results indicate that a reduced duration of pAPC migration after virus infection likely reduces the amplitude of the T<jats:sub>CD8<jats:sup>+<\/jats:sup><\/jats:sub>response, allowing persistence of the peripheral virus.<\/jats:p>","DOI":"10.1128\/jvi.01975-09","type":"journal-article","created":{"date-parts":[[2010,1,21]],"date-time":"2010-01-21T02:09:21Z","timestamp":1264039761000},"page":"3586-3594","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Dendritic Cell Migration Limits the Duration of CD8<sup>+<\/sup>T-Cell Priming to Peripheral Viral Antigen"],"prefix":"10.1128","volume":"84","author":[{"given":"Amanda M.","family":"Schell","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, MC H107, Penn State M. S. Hershey College of Medicine, Hershey, Pennsylvania 17033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica L.","family":"Granger","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, MC H107, Penn State M. S. Hershey College of Medicine, Hershey, Pennsylvania 17033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Koczot","sequence":"additional","affiliation":[{"name":"Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892-0440"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew A.","family":"Fischer","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, MC H107, Penn State M. S. Hershey College of Medicine, Hershey, Pennsylvania 17033"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher C.","family":"Norbury","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, MC H107, Penn State M. S. Hershey College of Medicine, Hershey, Pennsylvania 17033"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1087576"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.04.017"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200323630"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1724"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","first-page":"3270","DOI":"10.4049\/jimmunol.158.7.3270","volume":"158","year":"1997","unstructured":"Brossart, P., A. W. Goldrath, E. A. Butz, S. Martin, and M. J. Bevan. 1997. 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