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The nucleoprotein (NP) of the highly pathogenic arenavirus Jun\u00edn virus (JUNV) produces three N-terminally truncated isoforms: two (NP\n            <jats:sub>47kD<\/jats:sub>\n            and NP\n            <jats:sub>40kD<\/jats:sub>\n            ) are known to be produced by caspase cleavage, while, here, we show that NP\n            <jats:sub>53kD<\/jats:sub>\n            is produced by alternative translation initiation. Recombinant JUNVs lacking individual NP isoforms revealed that all three isoforms contribute to inhibiting caspase activation during infection, but cleavage to generate NP\n            <jats:sub>40kD<\/jats:sub>\n            makes the biggest contribution. Importantly, all three isoforms retain their ability to digest double-stranded (ds)RNA and inhibit interferon promoter activation but have a diffuse cytoplasmic distribution. 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