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Previously, our group reported that NS1 secretion in mosquito cells follows an unconventional secretion pathway dependent on caveolin-1. In this work, we demonstrate that in mosquito cells, but not in vertebrate cells, NS1 secretion takes place in association with the chaperone caveolin complex, a complex formed by caveolin-1 and the chaperones FKBP52, CyA, and Cy40, which are in charge of cholesterol transport inside the cell. Results obtained with ZIKV-infected mosquito cells suggest that ZIKV NS1 is released following an unconventional secretory route in association with the chaperone caveolin complex. These results uncover important differences in the virus-cell interactions between the vertebrate host and the mosquito vector, as well as novel functions for the chaperone caveolin complex. Moreover, manipulation of the NS1 secretory route may prove a valuable strategy to combat these two mosquito-borne diseases.<\/jats:p>","DOI":"10.1128\/jvi.01985-18","type":"journal-article","created":{"date-parts":[[2018,11,21]],"date-time":"2018-11-21T08:01:10Z","timestamp":1542787270000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["The Dengue Virus Nonstructural Protein 1 (NS1) Is Secreted from Mosquito Cells in Association with the Intracellular Cholesterol Transporter Chaperone Caveolin Complex"],"prefix":"10.1128","volume":"93","author":[{"given":"Romel","family":"Rosales Ramirez","sequence":"first","affiliation":[{"name":"Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan E.","family":"Ludert","sequence":"additional","affiliation":[{"name":"Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies (CINVESTAV-IPN), Mexico City, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60572-9"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature12060"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-800098-4.00001-5"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.03.008"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247749"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1017338108"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2015.11.020"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1600323"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.7.6104-6110.1999"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000881"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2122"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(00)00150-5"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90143-Z"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1124\/pr.54.3.431"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.3RU0317-090RR"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2005-6-7-226"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.2174\/1874467208666150519114115"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444902017523"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867033457160"},{"key":"e_1_3_2_21_2","doi-asserted-by":"crossref","unstructured":"Rosales Ramirez R Ludert JE. 2018. 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