{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T03:39:39Z","timestamp":1779248379780,"version":"3.51.4"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T00:00:00Z","timestamp":1584403200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T00:00:00Z","timestamp":1584403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000833","name":"Rosetrees Trust","doi-asserted-by":"publisher","award":["M914"],"award-info":[{"award-number":["M914"]}],"id":[{"id":"10.13039\/501100000833","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UK Research and Innovation | Medical Research Council","doi-asserted-by":"publisher","award":["G:0900466"],"award-info":[{"award-number":["G:0900466"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UK Research and Innovation | Medical Research Council","doi-asserted-by":"publisher","award":["MR\/RO21384\/1"],"award-info":[{"award-number":["MR\/RO21384\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UK Research and Innovation | Medical Research Council","doi-asserted-by":"publisher","award":["1486672"],"award-info":[{"award-number":["1486672"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2020,3,17]]},"abstract":"<jats:p>\n            Human cytomegalovirus (HCMV) is major pathogen of nonimmunocompetent individuals that remains in need of new therapeutic options. Here, we identify a potent antiviral compound (4,4\u2032-diisothiocyano-2,2\u2032-stilbenedisulfonic acid [DIDS]), its mechanism of action, and the chemical properties required for its activity. In doing so, the data argue that cysteine-reactive compounds could have the capacity to be developed for anti-HCMV activity. Importantly, the data show that entry of DIDS-resistant virus became independent of heparan sulfate proteoglycans (HSPGs) but, concomitantly, became more sensitive to neutralizing antibody responses. This serendipitous observation suggests that retention of an interaction with HSPGs during the entry process\n            <jats:italic>in vivo<\/jats:italic>\n            may be evolutionarily advantageous through better evasion of humoral responses directed against HCMV virions.\n          <\/jats:p>","DOI":"10.1128\/jvi.02012-19","type":"journal-article","created":{"date-parts":[[2020,1,14]],"date-time":"2020-01-14T13:16:40Z","timestamp":1579007800000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Evasion of a Human Cytomegalovirus Entry Inhibitor with Potent Cysteine Reactivity Is Concomitant with the Utilization of a Heparan Sulfate Proteoglycan-Independent Route of Entry"],"prefix":"10.1128","volume":"94","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0379-7161","authenticated-orcid":false,"given":"M. J.","family":"Murray","sequence":"first","affiliation":[{"name":"Institute of Immunity and Transplantation, UCL, Royal Free Hospital Campus, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. I.","family":"Bonilla-Medrano","sequence":"additional","affiliation":[{"name":"Institute of Immunity and Transplantation, UCL, Royal Free Hospital Campus, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Q. L.","family":"Lee","sequence":"additional","affiliation":[{"name":"Institute of Immunity and Transplantation, UCL, Royal Free Hospital Campus, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. J.","family":"Oxenford","sequence":"additional","affiliation":[{"name":"Translational Research Office, School of Pharmacy, UCL, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R.","family":"Angell","sequence":"additional","affiliation":[{"name":"Translational Research Office, School of Pharmacy, UCL, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. P.","family":"Depledge","sequence":"additional","affiliation":[{"name":"Department of Microbiology, New York University School of Medicine, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4909-1076","authenticated-orcid":false,"given":"M. B.","family":"Reeves","sequence":"additional","affiliation":[{"name":"Institute of Immunity and Transplantation, UCL, Royal Free Hospital Campus, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-74-2-255"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1192"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature07209"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1806305115"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006281"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0406821101"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.82"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/v10120704"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.06.028"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1814850116"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(92)90200-9"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034795"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0709704104"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12355"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.66.3.1761-1764.1992"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.63.1.52-58.1989"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302267200"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.20.12692-12702.2005"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01809-09"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1316517110"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1415310111"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00467-12"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1424818112"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1986.tb04606.x"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines5040039"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1814835116"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01794-16"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.106"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1993.1575"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.12.6688-6691.2004"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000882"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-9-2297"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01623-08"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01336-18"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.692673"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1006845"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1258758"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9008837"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(04)00183-2"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906798106"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1021\/jm3011433"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00568970"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.79888-0"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1992.tb17417.x"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1110\/ps.073397708"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040410"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01495-07"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/abbs\/gmp014"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01188"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI42955"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.031724"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1159\/000087236"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.107524.110"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.129684.111"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02012-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02012-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T15:06:33Z","timestamp":1646492793000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.02012-19"}},"subtitle":[],"editor":[{"given":"Richard M.","family":"Longnecker","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2020,3,17]]},"references-count":55,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,3,17]]}},"alternative-id":["10.1128\/JVI.02012-19"],"URL":"https:\/\/doi.org\/10.1128\/jvi.02012-19","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,17]]},"assertion":[{"value":"2019-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02012-19"}}