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The first-in-class HIV-1 maturation inhibitor bevirimat was highly effective in blocking HIV-1 replication, but its activity was compromised by naturally occurring sequence polymorphisms within Gag. Recently developed bevirimat analogs, referred to as \u201csecond-generation\u201d maturation inhibitors, overcome this issue. To understand more about how these second-generation compounds block HIV-1 maturation, here we selected for HIV-1 mutants that are resistant to these compounds. Selections were performed in the context of two different subtypes of HIV-1. We identified a small set of mutations at highly conserved positions within the capsid and spacer peptide 1 domains of Gag that confer resistance. Identification and analysis of these maturation inhibitor-resistant mutants provide insights into the mechanisms of resistance to these compounds.<\/jats:p>","DOI":"10.1128\/jvi.02017-18","type":"journal-article","created":{"date-parts":[[2018,12,20]],"date-time":"2018-12-20T06:12:46Z","timestamp":1545286366000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Resistance to Second-Generation HIV-1 Maturation Inhibitors"],"prefix":"10.1128","volume":"93","author":[{"given":"Emiko","family":"Urano","sequence":"first","affiliation":[{"name":"Virus-Cell Interaction Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uddhav","family":"Timilsina","sequence":"additional","affiliation":[{"name":"Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi, 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