{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T03:03:09Z","timestamp":1792810989834,"version":"4.5.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2020,3,17]],"date-time":"2020-03-17T00:00:00Z","timestamp":1584403200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000180","name":"U.S. Department of Homeland Security","doi-asserted-by":"publisher","award":["70RSAT19KPM000056"],"award-info":[{"award-number":["70RSAT19KPM000056"]}],"id":[{"id":"10.13039\/100000180","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000180","name":"U.S. Department of Homeland Security","doi-asserted-by":"publisher","award":["70RSAT18KPM000134"],"award-info":[{"award-number":["70RSAT18KPM000134"]}],"id":[{"id":"10.13039\/100000180","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2020,3,17]]},"abstract":"<jats:p>\n                    Currently, there is no commercially available vaccine against African swine fever. Outbreaks of this disease are devastating the swine industry from Central Europe to East Asia, and they are being caused by circulating strains of African swine fever virus derived from the Georgia 2007 isolate. Here, we report the discovery of a previously uncharacterized virus gene, which when deleted completely attenuates the Georgia isolate. Importantly, animals infected with this genetically modified virus were protected from developing ASF after challenge with the virulent parental virus. Interestingly, ASFV-G-\u0394I177L confers protection even at low doses (10\n                    <jats:sup>2<\/jats:sup>\n                    HAD\n                    <jats:sub>50<\/jats:sub>\n                    ) and remains completely attenuated when inoculated at high doses (10\n                    <jats:sup>6<\/jats:sup>\n                    HAD\n                    <jats:sub>50<\/jats:sub>\n                    ), demonstrating its potential as a safe vaccine candidate. At medium or higher doses (10\n                    <jats:sup>4<\/jats:sup>\n                    HAD\n                    <jats:sub>50<\/jats:sub>\n                    ), sterile immunity is achieved. Therefore, ASFV-G-\u0394I177L is a novel efficacious experimental ASF vaccine protecting pigs from the epidemiologically relevant ASFV Georgia isolate.\n                  <\/jats:p>","DOI":"10.1128\/jvi.02017-19","type":"journal-article","created":{"date-parts":[[2020,1,17]],"date-time":"2020-01-17T08:23:46Z","timestamp":1579249426000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":309,"title":["Development of a Highly Effective African Swine Fever Virus Vaccine by Deletion of the I177L Gene Results in Sterile Immunity against the Current Epidemic Eurasia Strain"],"prefix":"10.1128","volume":"94","author":[{"given":"Manuel V.","family":"Borca","sequence":"first","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Ramirez-Medina","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA"},{"name":"Department of Pathobiology and Veterinary Science, University of Connecticut, Storrs, Connecticut, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ediane","family":"Silva","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA"},{"name":"Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Vuono","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA"},{"name":"Department of Pathobiology and Population Medicine, Mississippi State University, Starkville, Mississippi, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayushi","family":"Rai","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA"},{"name":"Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Pruitt","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA"},{"name":"Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren G.","family":"Holinka","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauro","family":"Velazquez-Salinas","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA"},{"name":"Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Zhu","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas P.","family":"Gladue","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture, Agricultural Research Service, Plum Island Animal Disease Center, Greenport, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-68618-7_2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2009.0098"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1704.101283"},{"key":"e_1_3_2_5_2","first-page":"48","article-title":"African swine fever virus. Pathogenesis","volume":"18","author":"Coggins L","year":"1974","unstructured":"Coggins L. 1974. African swine fever virus. Pathogenesis. Prog Med Virol 18:48\u201363.","journal-title":"Prog Med Virol"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/bf01314703"},{"key":"e_1_3_2_7_2","volume-title":"Approaches to vaccination African swine fever","author":"Kihm UAM","year":"1987","unstructured":"Kihm UAM, Mueller H, Pool R. 1987. Approaches to vaccination African swine fever. Martinus Nijhoff Publishing, Boston, MA."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0065-3527(08)60714-9"},{"key":"e_1_3_2_9_2","first-page":"711","article-title":"Clinical and immunologic responses of pigs to African swine fever virus isolated from the Western Hemisphere","volume":"45","author":"Hamdy FM","year":"1984","unstructured":"Hamdy FM, Dardiri AH. 1984. Clinical and immunologic responses of pigs to African swine fever virus isolated from the Western Hemisphere. 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Inhibition of African swine fever infection in the presence of immune sera in vivo and in vitro. 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Temporal transcriptome and promoter architecture of the African swine fever virus. bioRxiv doi: https:\/\/doi.org\/10.1101\/847343.","DOI":"10.1101\/847343"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02017-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02017-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:05:40Z","timestamp":1646474740000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.02017-19"}},"subtitle":[],"editor":[{"given":"Joanna L.","family":"Shisler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,3,17]]},"references-count":27,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2020,3,17]]}},"alternative-id":["10.1128\/JVI.02017-19"],"URL":"https:\/\/doi.org\/10.1128\/jvi.02017-19","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/861666","asserted-by":"object"}]},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,3,17]]},"assertion":[{"value":"2019-11-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-01-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02017-19"}}