{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:36:46Z","timestamp":1789432606726,"version":"build-2803163510"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Friend virus induces an erythroleukemia in susceptible mice that is initiated by the interaction of the Friend virus-encoded glycoprotein gp55 with the erythropoietin (Epo) receptor and the product of the host\n            <jats:italic>Fv2<\/jats:italic>\n            gene, a naturally occurring truncated form of the Stk receptor tyrosine kinase (Sf-Stk). We have previously demonstrated that the activation of Sf-Stk, recruitment of a Grb2\/Gab2\/Stat3 signaling complex, and induction of Pu.1 expression by Stat3 are required for the development of the early stage of Friend disease both\n            <jats:italic>in vitro<\/jats:italic>\n            and\n            <jats:italic>in vivo<\/jats:italic>\n            . Here we demonstrate that the interaction of gp55 with Sf-Stk is dependent on cysteine residues in the ecotropic domain of gp55 and the extracellular domain of Sf-Stk. Point mutation of these cysteine residues or deletion of these domains inhibits the ability of gp55 to interact with Sf-Stk, resulting in the inability of these proteins to promote the Epo-independent growth of erythroid progenitor cells. We also demonstrate that the interaction of gp55 with Sf-Stk does not promote dimerization of Sf-Stk but results in enhanced phosphorylation of Sf-Stk and the relocalization of Sf-Stk from the cytosol to the plasma membrane. Finally, we demonstrate that a constitutively active form of Sf-Stk (Sf-StkM330T), as well as its human counterpart, Sf-Ron, promotes Epo-independent colony formation in the absence of gp55 and that this response is also dependent on the cysteines in the extracellular domains of Sf-StkM330T and Sf-Ron. These data suggest that the cysteines in the extracellular domains of Sf-Stk and Sf-Ron may also mediate the interaction of these truncated receptors with other cellular factors that regulate their ability to promote cytokine-independent growth.\n          <\/jats:p>","DOI":"10.1128\/jvi.02090-09","type":"journal-article","created":{"date-parts":[[2009,12,17]],"date-time":"2009-12-17T04:34:52Z","timestamp":1261024492000},"page":"2223-2235","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Activation of the N-Terminally Truncated Form of the Stk Receptor Tyrosine Kinase Sf-Stk by Friend Virus-Encoded gp55 Is Mediated by Cysteine Residues in the Ecotropic Domain of gp55 and the Extracellular Domain of Sf-Stk"],"prefix":"10.1128","volume":"84","author":[{"given":"Shihan","family":"He","sequence":"first","affiliation":[{"name":"Pathobiology Graduate Program"},{"name":"Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuang","family":"Ni","sequence":"additional","affiliation":[{"name":"Immunology and Infectious Disease Graduate Program"},{"name":"Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shailaja","family":"Hegde","sequence":"additional","affiliation":[{"name":"Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Cell and Developmental Biology Graduate Program"},{"name":"Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"Sharda","sequence":"additional","affiliation":[{"name":"Pathobiology Graduate Program"},{"name":"Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avery","family":"August","sequence":"additional","affiliation":[{"name":"Pathobiology Graduate Program"},{"name":"Immunology and Infectious Disease Graduate Program"},{"name":"Cell and Developmental Biology Graduate Program"},{"name":"Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert F.","family":"Paulson","sequence":"additional","affiliation":[{"name":"Pathobiology Graduate Program"},{"name":"Immunology and Infectious Disease Graduate Program"},{"name":"Cell and Developmental Biology Graduate Program"},{"name":"Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pamela A.","family":"Hankey","sequence":"additional","affiliation":[{"name":"Pathobiology Graduate Program"},{"name":"Immunology and Infectious Disease Graduate Program"},{"name":"Cell and Developmental Biology Graduate Program"},{"name":"Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania 16802"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.50.3.813-821.1984"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1990.tb07379.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.80.13.3913"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0600"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90428-2"},{"key":"e_1_3_2_7_2","first-page":"179","volume":"3","year":"1988","unstructured":"Ben-David, Y., V. 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