{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:15:37Z","timestamp":1790914537911,"version":"4.1.0"},"reference-count":65,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2016,1,15]],"date-time":"2016-01-15T00:00:00Z","timestamp":1452816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100005825","name":"USDA | National Institute of Food and Agriculture","doi-asserted-by":"crossref","award":["2013-67015-21311"],"award-info":[{"award-number":["2013-67015-21311"]}],"id":[{"id":"10.13039\/100005825","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["P30-GM110703"],"award-info":[{"award-number":["P30-GM110703"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"crossref","award":["P20GM103433"],"award-info":[{"award-number":["P20GM103433"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2016,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The immediate early 62 protein (IE62) of varicella-zoster virus (VZV), a major viral\n            <jats:italic>trans<\/jats:italic>\n            -activator, initiates the virus life cycle and is a key component of pathogenesis. The IE62 possesses several domains essential for\n            <jats:italic>trans<\/jats:italic>\n            -activation, including an acidic\n            <jats:italic>trans<\/jats:italic>\n            -activation domain (TAD), a serine-rich tract (SRT), and binding domains for USF, TFIIB, and TATA box binding protein (TBP). Transient-transfection assays showed that the VZV IE62 lacking the SRT\n            <jats:italic>trans<\/jats:italic>\n            -activated the early VZV ORF61 promoter at only 16% of the level of the full-length IE62. When the SRT of IE62 was replaced with the SRT of equine herpesvirus 1 (EHV-1) IEP, its\n            <jats:italic>trans<\/jats:italic>\n            -activation activity was completely restored. Herpes simplex virus 1 (HSV-1) ICP4 that lacks a TAD very weakly (1.5-fold)\n            <jats:italic>trans<\/jats:italic>\n            -activated the ORF61 promoter. An IE62 TAD-ICP4 chimeric protein exhibited\n            <jats:italic>trans<\/jats:italic>\n            -activation ability (10.2-fold), indicating that the IE62 TAD functions with the SRT of HSV-1 ICP4 to\n            <jats:italic>trans<\/jats:italic>\n            -activate viral promoters. When the serine and acidic residues of the SRT were replaced with Ala, Leu, and Gly,\n            <jats:italic>trans<\/jats:italic>\n            -activation activities of the modified IE62 proteins IE62-SRT\u0394Se and IE62-SRT\u0394Ac were reduced to 46% and 29% of wild-type activity, respectively. Bimolecular complementation assays showed that the TAD of IE62, EHV-1 IEP, and HSV-1 VP16 interacted with Mediator 25 in human melanoma MeWo cells. The SRT of IE62 interacted with the nucleolar-ribosomal protein EAP, which resulted in the formation of globular structures within the nucleus. These results suggest that the SRT plays an important role in VZV viral gene expression and replication.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            The immediate early 62 protein (IE62) of varicella-zoster virus (VZV) is a major viral\n            <jats:italic>trans<\/jats:italic>\n            -activator and is essential for viral growth. Our data show that the serine-rich tract (SRT) of VZV IE62, which is well conserved within the alphaherpesviruses, is needed for\n            <jats:italic>trans<\/jats:italic>\n            -activation mediated by the acidic\n            <jats:italic>trans<\/jats:italic>\n            -activation domain (TAD). The TADs of IE62, EHV-1 IEP, and HSV-1 VP16 interacted with cellular Mediator 25 in bimolecular complementation assays. The interaction of the IE62 SRT with nucleolar-ribosomal protein EAP resulted in the formation of globular structures within the nucleus. Understanding the mechanisms by which the TAD and SRT of IE62 contribute to the function of this essential regulatory protein is important in understanding the gene program of this human pathogen.\n          <\/jats:p>","DOI":"10.1128\/jvi.02096-15","type":"journal-article","created":{"date-parts":[[2015,11,5]],"date-time":"2015-11-05T03:43:32Z","timestamp":1446695012000},"page":"959-971","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Functional Characterization of the Serine-Rich Tract of Varicella-Zoster Virus IE62"],"prefix":"10.1128","volume":"90","author":[{"given":"Seong K.","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, and Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akhalesh K.","family":"Shakya","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, and Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seongman","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, and Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dennis J.","family":"O'Callaghan","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, and Center for Molecular and Tumor Virology, Louisiana State University Health Sciences Center, Shreveport, Louisiana, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"2773","volume-title":"Fields virology","author":"Cohen JI","year":"2007","unstructured":"Cohen JI, Straus S, Arvin A. 2007. 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