{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T00:06:07Z","timestamp":1793232367540,"version":"4.5.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2019,4,1]],"date-time":"2019-04-01T00:00:00Z","timestamp":1554076800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003130","name":"Fonds Wetenschappelijk Onderzoek","doi-asserted-by":"crossref","award":["G.0176.15"],"award-info":[{"award-number":["G.0176.15"]}],"id":[{"id":"10.13039\/501100003130","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Special Research Grant of Ghent University","award":["01G01317"],"award-info":[{"award-number":["01G01317"]}]},{"DOI":"10.13039\/501100003132","name":"Agentschap voor Innovatie door Wetenschap en Technologie","doi-asserted-by":"publisher","award":["PhD grant"],"award-info":[{"award-number":["PhD grant"]}],"id":[{"id":"10.13039\/501100003132","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002749","name":"Federaal Wetenschapsbeleid","doi-asserted-by":"publisher","award":["IAP phase VII BELVIR"],"award-info":[{"award-number":["IAP phase VII BELVIR"]}],"id":[{"id":"10.13039\/501100002749","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>Natural killer (NK) cells display a prominent cytolytic activity against virus-infected cells and are indispensable in the innate antiviral response, particularly against herpesviruses. Despite their importance in the control of alphaherpesvirus infections, relatively little is known about the mechanisms that trigger NK cell cytotoxicity against alphaherpesvirus-infected cells. Here, using the porcine alphaherpesvirus pseudorabies virus (PRV), we found that the conserved alphaherpesvirus glycoprotein gB triggers NK cell-mediated cytotoxicity, both in virus-infected and in gB-transfected cells. In addition, we report that gB expression results in increased cell surface binding of porcine paired immunoglobulin-like type 2 receptor beta (PILR\u03b2), an activating NK cell receptor. The interaction between PILR\u03b2 and viral gB may have consequences that stretch beyond the interaction with NK cells, including virus entry into host cells. The identification of gB as an NK cell-activating viral protein may be of importance in the construction of future vaccines and therapeutics requiring optimized interactions of alphaherpesviruses with NK cells.<\/jats:p>","DOI":"10.1128\/jvi.02107-18","type":"journal-article","created":{"date-parts":[[2019,1,25]],"date-time":"2019-01-25T12:55:19Z","timestamp":1548420919000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":14,"title":["Expression of the Pseudorabies Virus gB Glycoprotein Triggers NK Cell Cytotoxicity and Increases Binding of the Activating NK Cell Receptor PILR\u03b2"],"prefix":"10.1128","volume":"93","author":[{"given":"Steffi","family":"De Pelsmaeker","sequence":"first","affiliation":[{"name":"Laboratory of Immunology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelien","family":"Dierick","sequence":"additional","affiliation":[{"name":"Department of Pathology, Bacteriology and Poultry Diseases, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Klupp","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Institute of Molecular Virology and Cell Biology, Greifswald-Insel Riems, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas C.","family":"Mettenleiter","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Institute of Molecular Virology and Cell Biology, Greifswald-Insel Riems, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Cantoni","sequence":"additional","affiliation":[{"name":"Department of Experimental Medicine and Center of Excellence for Biomedical Research, University of Genoa and IRCCS Instituto Giannina Gaslini, Genoa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Massimo","family":"Vitale","sequence":"additional","affiliation":[{"name":"UOC Immunologia, Ospedale Policlinico San Martino, Genoa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herman W.","family":"Favoreel","sequence":"additional","affiliation":[{"name":"Laboratory of Immunology, Department of Virology, Parasitology and Immunology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2015.10.008"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2004.11.022"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/039463201102400128"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2013.07.020"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni1582"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2014.02.001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1409485111"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02902-15"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2016.02.007"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri3174"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-77-5-987"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.157.5.1544"},{"key":"e_1_3_2_14_2","doi-asserted-by":"crossref","first-page":"2206","DOI":"10.4049\/jimmunol.133.4.2206","article-title":"Human natural killer cell recognition of herpes simplex virus type 1 glycoproteins: specificity analysis with the use of monoclonal antibodies and antigenic variants","volume":"133","author":"Bishop GA","year":"1984","unstructured":"Bishop GA, Marlin SD, Schwartz SA, Glorioso JC. 1984. Human natural killer cell recognition of herpes simplex virus type 1 glycoproteins: specificity analysis with the use of monoclonal antibodies and antigenic variants. J Immunol 133:2206\u20132214.","journal-title":"J Immunol"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.15.7137-7145.2000"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.10.5279-5287.2004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.01.043"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.3.1686"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01039-10"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1320716111"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02601-08"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00284.2005"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.3.1197"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031885"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1086\/512862"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2007.03.005"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/cti2.1010"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201646762"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029538"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029488"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a029512"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2015.07.004"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3013"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(59)90068-6"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.13.6845-6851.2002"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.4A0417-163RR"},{"key":"e_1_3_2_37_2","doi-asserted-by":"crossref","first-page":"368","DOI":"10.4049\/jimmunol.133.1.368","article-title":"Preparation and characterization of monoclonal antibodies reactive with porcine PBL","volume":"133","author":"Pescovitz MD","year":"1984","unstructured":"Pescovitz MD, Lunney JK, Sachs DH. 1984. Preparation and characterization of monoclonal antibodies reactive with porcine PBL. J Immunol 133:368\u2013375.","journal-title":"J Immunol"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2567.1996.d01-682.x"},{"key":"e_1_3_2_39_2","doi-asserted-by":"crossref","first-page":"647","DOI":"10.4049\/jimmunol.133.2.647","article-title":"Monoclonal antibodies reactive with swine lymphocytes. I. Antibodies to membrane structures that define the cytolytic T lymphocyte subset in the swine","volume":"133","author":"Jonji\u0107 S","year":"1984","unstructured":"Jonji\u0107 S, Koszinowski UH. 1984. Monoclonal antibodies reactive with swine lymphocytes. I. Antibodies to membrane structures that define the cytolytic T lymphocyte subset in the swine. J Immunol 133:647\u2013652.","journal-title":"J Immunol"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01315422"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02107-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02107-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T05:38:58Z","timestamp":1694583538000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.02107-18"}},"subtitle":[],"editor":[{"given":"Richard M.","family":"Longnecker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,4]]},"references-count":39,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2019,4]]}},"alternative-id":["10.1128\/JVI.02107-18"],"URL":"https:\/\/doi.org\/10.1128\/jvi.02107-18","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4]]},"assertion":[{"value":"2018-11-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-01-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-03-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02107-18"}}