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Accumulation of\nthe viral envelope proteins at this compartment is a prerequisite for\nvirus assembly. Previously, we reported the identification of a dibasic\nmotif (KxHxx) in the cytoplasmic tail of the SARS CoV spike (S) protein\nthat was similar to a canonical dilysine ER retrieval signal. Here we\ndemonstrate that this motif is a novel and functional ER retrieval\nsignal which reduced the rate of traffic of the full-length S protein\nthrough the Golgi complex. The KxHxx motif also partially retained two\ndifferent reporter proteins in the ERGIC region and reduced their rates\nof trafficking, although the motif was less potent than the canonical\ndilysine signal. The dibasic motif bound the coatomer complex I (COPI)\nin an in vitro binding assay, suggesting that ER retrieval may\ncontribute to the accumulation of SARS CoV S protein near the virus\nassembly site for interaction with other viral structural proteins. In\nsupport of this, we found that the dibasic motif on the SARS S protein\nwas required for its localization to the ERGIC\/Golgi region when\ncoexpressed with SARS membrane (M) protein. Thus, the cycling of SARS S\nthrough the ER-Golgi system may be required for its incorporation into\nassembling virions in the\nERGIC.<\/jats:p>","DOI":"10.1128\/jvi.02146-06","type":"journal-article","created":{"date-parts":[[2006,12,14]],"date-time":"2006-12-14T02:53:03Z","timestamp":1166064783000},"page":"2418-2428","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":200,"title":["The Cytoplasmic Tail of the Severe Acute Respiratory Syndrome Coronavirus Spike Protein Contains a Novel Endoplasmic Reticulum Retrieval Signal That Binds COPI and Promotes Interaction with Membrane Protein"],"prefix":"10.1128","volume":"81","author":[{"given":"Corrin E.","family":"McBride","sequence":"first","affiliation":[{"name":"Department of Cell Biology, The Johns Hopkins University School of Medicine,\rBaltimore, Maryland 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, The Johns Hopkins University School of Medicine,\rBaltimore, Maryland 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn E.","family":"Machamer","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, The Johns Hopkins University School of Medicine,\rBaltimore, Maryland 21205"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.21.15080"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.11.8636-8643.1998"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0401939101"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.02.001"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400576101"},{"key":"e_1_3_2_7_2","first-page":"73","year":"1995","unstructured":"Cavanagh, D. 1995. 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