{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T15:24:22Z","timestamp":1790349862257,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2019,3,15]],"date-time":"2019-03-15T00:00:00Z","timestamp":1552608000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Natural Sciences and Research Council","award":["90887-2010 RGPIN"],"award-info":[{"award-number":["90887-2010 RGPIN"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2019,3,15]]},"abstract":"<jats:p>\n                    Nuclear localization signals (NLSs) and nuclear export signals (NESs) are important elements directing VP8 to the desired locations in the BoHV-1-infected cell. In this study, a critical regulator that switches the nuclear and cytoplasmic localization of VP8 in BoHV-1-infected cells was identified. BoHV-1 used viral kinase US3 to regulate the cellular localization of VP8. Early during BoHV-1 infection VP8 was localized in the nucleus, where it performs various functions; once US3 was expressed, phosphorylated VP8 was cytoplasmic and ultimately accumulated in the\n                    <jats:italic>cis<\/jats:italic>\n                    -Golgi apparatus, presumably to be incorporated into virions. The Golgi localization of VP8 was only observed in virus-infected cells and not in US3-cotransfected cells, suggesting that this is mediated by other viral factors. Interestingly, VP8 was shown to cause increased cholesterol levels, which is a novel function for VP8 and a potential strategy to supply lipid for viral replication.\n                  <\/jats:p>","DOI":"10.1128\/jvi.02151-18","type":"journal-article","created":{"date-parts":[[2019,1,7]],"date-time":"2019-01-07T17:00:29Z","timestamp":1546880429000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["US3 Kinase-Mediated Phosphorylation of Tegument Protein VP8 Plays a Critical Role in the Cellular Localization of VP8 and Its Effect on the Lipid Metabolism of Bovine Herpesvirus 1-Infected Cells"],"prefix":"10.1128","volume":"93","author":[{"given":"Kuan","family":"Zhang","sequence":"first","affiliation":[{"name":"VIDO-InterVac, University of Saskatchewan, Saskatoon, Saskatchewan, Canada"},{"name":"Vaccinology and Immunotherapeutics, University of Saskatchewan, Saskatoon, Saskatchewan, 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