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Here, we identify serine\/threonine phosphorylation sites for cellular and viral kinases within pUL69. We demonstrate that the pUL97\/CDK phosphosites within alpha-helix 2 of pUL69 are crucial for its\n            <jats:italic>cis\/trans<\/jats:italic>\n            isomerization by the cellular protein Pin1. Thus, we identified pUL69 as the first HCMV-encoded protein that is phosphorylated by cellular and viral serine\/threonine kinases in order to serve as a substrate for Pin1. Furthermore, our study revealed that betaherpesviral mRNA export proteins contain extended binding motifs for the cellular mRNA adaptor proteins UAP56\/URH49 harboring phosphorylated serines that are critical for efficient viral replication. Knowledge of the phosphorylation sites of pUL69 and the processes regulated by these posttranslational modifications is important in order to develop antiviral strategies based on a specific interference with pUL69 phosphorylation.\n          <\/jats:p>","DOI":"10.1128\/jvi.02151-19","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T09:34:53Z","timestamp":1579685693000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":13,"title":["Phosphosite Analysis of the Cytomegaloviral mRNA Export Factor pUL69 Reveals Serines with Critical Importance for Recruitment of Cellular Proteins Pin1 and UAP56\/URH49"],"prefix":"10.1128","volume":"94","author":[{"given":"Marco","family":"Thomas","sequence":"first","affiliation":[{"name":"Institute of Clinical and Molecular Virology, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg (FAU), Erlangen-Nuremberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Regina","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Molecular Virology, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg (FAU), Erlangen-Nuremberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Horn","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Molecular Virology, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg (FAU), Erlangen-Nuremberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boris","family":"Bogdanow","sequence":"additional","affiliation":[{"name":"Max Delbrueck Center for Molecular Medicine, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koshi","family":"Imami","sequence":"additional","affiliation":[{"name":"Max Delbrueck Center for Molecular Medicine, Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Milbradt","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Molecular Virology, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg (FAU), Erlangen-Nuremberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mirjam","family":"Steingruber","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Molecular Virology, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg (FAU), Erlangen-Nuremberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manfred","family":"Marschall","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Molecular Virology, Friedrich-Alexander-Universit\u00e4t Erlangen-N\u00fcrnberg (FAU), Erlangen-Nuremberg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva-Maria","family":"Schilling","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital Ulm, Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Torgils","family":"Fossen","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Bergen, Bergen, Norway"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Stamminger","sequence":"additional","affiliation":[{"name":"Institute for Virology, University Hospital Ulm, Ulm, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"2","article-title":"Nuclear export of herpes virus RNA","volume":"259","author":"Sandri-Goldin RM","year":"2001","unstructured":"Sandri-Goldin RM. 2001. 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