{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T17:39:41Z","timestamp":1790530781095,"version":"4.1.0"},"reference-count":69,"publisher":"American Society for Microbiology","issue":"13","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Initial Training Network Manifold","award":["317371"],"award-info":[{"award-number":["317371"]}]},{"name":"Marie Curie Action","award":["PCIG-GA-2011-294196"],"award-info":[{"award-number":["PCIG-GA-2011-294196"]}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["GRK 1949"],"award-info":[{"award-number":["GRK 1949"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2019,7]]},"abstract":"<jats:p>Poxviruses are large DNA viruses that can infect a wide range of host species. A number of these viruses are clinically important to humans, including variola virus (smallpox) and vaccinia virus. Since the eradication of smallpox, zoonotic infections with monkeypox virus and cowpox virus are emerging. Additionally, poxviruses can be engineered to specifically target cancer cells and are used as a vaccine vector against tuberculosis, influenza, and coronaviruses. Poxviruses rely on host factors for most stages of their life cycle, including attachment to the cell and entry. These host factors are crucial for virus infectivity and host cell tropism. We used a genome-wide knockout library of host cells to identify host factors necessary for vaccinia virus infection. We confirm a dominant role for heparin sulfate in mediating virus attachment. Additionally, we show that TMED10, previously not implicated in virus infections, facilitates virus uptake by modulating the cellular response to phosphatidylserine.<\/jats:p>","DOI":"10.1128\/jvi.02160-18","type":"journal-article","created":{"date-parts":[[2019,4,16]],"date-time":"2019-04-16T10:53:06Z","timestamp":1555411986000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["A Genome-Wide Haploid Genetic Screen Identifies Heparan Sulfate-Associated Genes and the Macropinocytosis Modulator TMED10 as Factors Supporting Vaccinia Virus Infection"],"prefix":"10.1128","volume":"93","author":[{"given":"Rutger D.","family":"Luteijn","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ferdy","family":"van Diemen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vincent A.","family":"Blomen","sequence":"additional","affiliation":[{"name":"Netherlands Cancer Institute, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid G. J.","family":"Boer","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saravanan","family":"Manikam Sadasivam","sequence":"additional","affiliation":[{"name":"Department of Membrane Biochemistry and Biophysics, Utrecht University, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toin H.","family":"van Kuppevelt","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Radboud University Medical Center, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingo","family":"Drexler","sequence":"additional","affiliation":[{"name":"Institute for Virology, Universit\u00e4tsklinikum D\u00fcsseldorf, Heinrich Heine University, D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thijn R.","family":"Brummelkamp","sequence":"additional","affiliation":[{"name":"Netherlands Cancer Institute, Amsterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert Jan","family":"Lebbink","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuel J.","family":"Wiertz","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00856-9"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1407647"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/QCO.0b013e3282f44c74"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(11)60694-6"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3121\/cmr.1.2.87"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(96)00195-8"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/v6072735"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad1283"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(14)70963-6"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiu528"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.03.021"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02331"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.262668999"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a010199"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.3390\/v4050688"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2011.11.007"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1006594107"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.05446-11"},{"key":"e_1_3_3_20_2","doi-asserted-by":"crossref","unstructured":"Luteijn RD van Diemen F Blomen VA Boer IGJ Manikam Sadasivam S van Kuppevelt T Drexler I Brummelkamp TR Lebbink RJ Wiertz EJ. 2018. A genome-wide haploid genetic screen for essential factors in vaccinia virus infection identifies TMED10 as regulator of macropinocytosis. bioRxiv https:\/\/doi.org\/10.1101\/493205.","DOI":"10.1101\/493205"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1369\/0022155412464972"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1233675"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aac7557"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M302439200"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109316200"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02055-15"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.39.25117"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2016.00015"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00432-17"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2008.12.029"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2012.08.044"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00599-10"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3469"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0509-510"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2354"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1155164"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2011.03.012"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909376106"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1004618107"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.16.8318-8334.2002"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature10348"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1252480"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.72.2.1577-1585.1998"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2009.04.012"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.7.3353-3365.2000"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.80831-0"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02302-06"},{"key":"e_1_3_3_48_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature16465"},{"key":"e_1_3_3_49_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e02-11-0724"},{"key":"e_1_3_3_50_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.030163"},{"key":"e_1_3_3_51_2","doi-asserted-by":"publisher","DOI":"10.1086\/657322"},{"key":"e_1_3_3_52_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00385"},{"key":"e_1_3_3_53_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.135.5.1239"},{"key":"e_1_3_3_54_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.29.17183"},{"key":"e_1_3_3_55_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.140.4.751"},{"key":"e_1_3_3_56_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.21.11393"},{"key":"e_1_3_3_57_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2008.00784.x"},{"key":"e_1_3_3_58_2","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2011.44.6.369"},{"key":"e_1_3_3_59_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M107150200"},{"key":"e_1_3_3_60_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e09-08-0735"},{"key":"e_1_3_3_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.05.017"},{"key":"e_1_3_3_62_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.01.028"},{"key":"e_1_3_3_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01157-06"},{"key":"e_1_3_3_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.07.005"},{"key":"e_1_3_3_65_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.07.014"},{"key":"e_1_3_3_66_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1112"},{"key":"e_1_3_3_67_2","doi-asserted-by":"publisher","DOI":"10.1038\/37022"},{"key":"e_1_3_3_68_2","doi-asserted-by":"publisher","DOI":"10.1385\/1-59259-789-0:077"},{"key":"e_1_3_3_69_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4832"},{"key":"e_1_3_3_70_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02531998"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02160-18","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02160-18","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T14:50:04Z","timestamp":1646491804000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.02160-18"}},"subtitle":[],"editor":[{"given":"Joanna L.","family":"Shisler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,7]]},"references-count":69,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2019,7]]}},"alternative-id":["10.1128\/JVI.02160-18"],"URL":"https:\/\/doi.org\/10.1128\/jvi.02160-18","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,7]]},"assertion":[{"value":"2018-12-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-04-11","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-06-14","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02160-18"}}