{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T15:22:14Z","timestamp":1767453734022},"reference-count":64,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2012,12,15]],"date-time":"2012-12-15T00:00:00Z","timestamp":1355529600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2012,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>CD8<jats:sup>+<\/jats:sup>T cells play an important role in protection against both acute and persistent viral infections, and new vaccines that induce CD8<jats:sup>+<\/jats:sup>T cell immunity are currently needed. Here, we show that lymphocytic choriomeningitis virus (LCMV)-specific CD8<jats:sup>+<\/jats:sup>T cells can be generated in response to a nonreplicating H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-inactivated whole-virus vaccine (H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-LCMV). Vaccine-induced CD8<jats:sup>+<\/jats:sup>T cell responses exhibited an increased ability to produce multiple cytokines at early time points following immunization compared to infection-induced responses. Vaccination with H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-LCMV induced the expansion of a narrow subset of the antigen-specific CD8<jats:sup>+<\/jats:sup>T cells induced by LCMV strain Arm infection, resulting in a distinct immunodominance hierarchy. Acute LCMV infection stimulated immunodominance patterns that shifted over time or after secondary infection, whereas vaccine-generated immunodominance profiles remained remarkably stable even following subsequent viral infection. Vaccine-induced CD8<jats:sup>+<\/jats:sup>T cell populations expanded sharply in response to challenge and were then maintained at high levels, with responses to individual epitopes occupying up to 40% of the CD8<jats:sup>+<\/jats:sup>T cell compartment at 35 days after challenge. H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-LCMV vaccination protected animals against challenge with chronic LCMV clone 13, and protection was mediated by CD8<jats:sup>+<\/jats:sup>T cells. These results indicate that vaccination with an H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>-inactivated whole-virus vaccine induces LCMV-specific CD8<jats:sup>+<\/jats:sup>T cells with unique functional characteristics and provides a useful model for studying CD8<jats:sup>+<\/jats:sup>T cells elicited in the absence of active viral infection.<\/jats:p>","DOI":"10.1128\/jvi.02178-12","type":"journal-article","created":{"date-parts":[[2012,10,11]],"date-time":"2012-10-11T13:01:29Z","timestamp":1349960489000},"page":"13735-13744","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Characterization of CD8<sup>+<\/sup>T Cell Function and Immunodominance Generated with an H<sub>2<\/sub>O<sub>2<\/sub>-Inactivated Whole-Virus Vaccine"],"prefix":"10.1128","volume":"86","author":[{"given":"Joshua M.","family":"Walker","sequence":"first","affiliation":[{"name":"Oregon National Primate Research Center, Oregon Health &amp; Sciences University, Beaverton, Oregon, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hans-Peter","family":"Rau\u00e9","sequence":"additional","affiliation":[{"name":"Oregon National Primate Research Center, Oregon Health &amp; Sciences University, Beaverton, Oregon, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark K.","family":"Slifka","sequence":"additional","affiliation":[{"name":"Oregon National Primate Research Center, Oregon Health &amp; Sciences University, Beaverton, Oregon, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.62.6.2102-2106.1988"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.160.2.521"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4161\/hv.4.4.5751"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2763"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.70.2.203"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/prca.200780009"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.7.4627"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-12-4818"},{"key":"e_1_3_2_10_2","first-page":"103","article-title":"Review of accidents caused by incomplete inactivation of viruses","volume":"81","author":"Brown F","year":"1993","unstructured":"BrownF. 1993. 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