{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T07:55:15Z","timestamp":1790495715387,"version":"4.1.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A newly discovered virally encoded deubiquitinating enzyme (DUB) is strictly conserved across the\n                    <jats:italic>Herpesviridae<\/jats:italic>\n                    . Epstein-Barr virus (EBV) BPLF1 encodes a tegument protein (3,149 amino acids) that exhibits deubiquitinating (DUB) activity that is lost upon mutation of the active-site cysteine. However, targets for the herpesviral DUBs have remained elusive. To investigate a predicted interaction between EBV BPLF1 and EBV ribonucleotide reductase (RR), a functional clone of the first 246 N-terminal amino acids of BPLF1 (BPLF1 1-246) was constructed. Immunoprecipitation verified an interaction between the small subunit of the viral RR2 and BPLF1 proteins. In addition, the large subunit (RR1) of the RR appeared to be ubiquitinated both in vivo and in vitro; however, ubiquitinated forms of the small subunit, RR2, were not detected. Ubiquitination of RR1 requires the expression of both subunits of the RR complex. Furthermore, coexpression of RR1 and RR2 with BPLF1 1-246 abolishes ubiquitination of RR1. EBV RR1, RR2, and BPLF1 1-246 colocalized to the cytoplasm in HEK 293T cells. Finally, expression of enzymatically active BPLF1 1-246 decreased RR activity, whereas a nonfunctional active-site mutant (BPLF1 C61S) had no effect. These results indicate that the EBV deubiquitinating enzyme interacts with, deubiquitinates, and influences the activity of the EBV RR. This is the first verified protein target of the EBV deubiquitinating enzyme.\n                  <\/jats:p>","DOI":"10.1128\/jvi.02195-08","type":"journal-article","created":{"date-parts":[[2009,2,25]],"date-time":"2009-02-25T20:55:21Z","timestamp":1235595321000},"page":"4345-4353","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["The Epstein-Barr Virus (EBV) Deubiquitinating Enzyme BPLF1 Reduces EBV Ribonucleotide Reductase Activity"],"prefix":"10.1128","volume":"83","author":[{"given":"Christopher B.","family":"Whitehurst","sequence":"first","affiliation":[{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunbin","family":"Ning","sequence":"additional","affiliation":[{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gretchen L.","family":"Bentz","sequence":"additional","affiliation":[{"name":"Departments of Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florent","family":"Dufour","sequence":"additional","affiliation":[{"name":"Centre de Recherche du Centre Hospitalier de l'Universite\u0301 de Montre\u0301al and Institut du Cancer de Montre\u0301al, Ho\u0302pital Notre-Dame, Montre\u0301al, Quebec H2L 4M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Gershburg","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Immunology, and Cell Biology, Southern Illinois University School of Medicine, Springfield, Illinois 62794"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Shackelford","sequence":"additional","affiliation":[{"name":"Cell and Developmental Biology, Lineberger Comprehensive Cancer Center, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Langelier","sequence":"additional","affiliation":[{"name":"Centre de Recherche du Centre Hospitalier de l'Universite\u0301 de Montre\u0301al and Institut du Cancer de Montre\u0301al, Ho\u0302pital Notre-Dame, Montre\u0301al, Quebec H2L 4M1, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph S.","family":"Pagano","sequence":"additional","affiliation":[{"name":"Departments of Medicine"},{"name":"Microbiology and Immunology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00270-07"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.45.1.397-407.1983"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1424"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00475a007"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00280-08"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702332104"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-69-10-2607"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0330774100"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb0805-758"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/321441a0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.14.8245"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.24.11608-11618.2000"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/321439a0"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)39337-7"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1988.tb02987.x"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1984.tb01897.x"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.2165\/00066982-200106040-00009"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.20238"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0700548104"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(88)90144-4"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01149-07"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.425"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.37.2.580-588.1981"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.67.1.71"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2005.07.003"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1436"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-83-11-2779"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2007.02.012"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-008-8090-6"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(88)90421-7"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.3.1435-1449.2002"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2091-9-7"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.09.019"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.24.15582-15585.2005"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1023\/B:VIRU.0000036387.39937.9b"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.24.12.5089-5093.2004"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.2741\/1738"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1006\/viro.1997.8645"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0854.2007.00610.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00686113"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/0065-2571(91)90009-B"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00401-06"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.11.14.9409543"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2530630"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02195-08","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02195-08","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:57:25Z","timestamp":1646467045000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.02195-08"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,5]]},"references-count":44,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2009,5]]}},"alternative-id":["10.1128\/JVI.02195-08"],"URL":"https:\/\/doi.org\/10.1128\/jvi.02195-08","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1158236.618361","asserted-by":"object"}]},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,5]]},"assertion":[{"value":"2008-10-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-02-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}