{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T08:49:20Z","timestamp":1789980560628,"version":"4.0.1"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T00:00:00Z","timestamp":1358208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Human immunodeficiency virus type 1 (HIV-1) transmission results from infection with one or a small number of variants from the donor quasispecies. Transmitted\/founder (T\/F) viruses have recently been identified from acutely infected patients, but the way in which they interact with primary targets of HIV-1 infection is poorly understood. We have conducted a biological characterization of a panel of subtype B T\/F acute and chronic envelope (Env)-expressing chimeric virus in primary human target cells and mucosal tissues. Both acute and chronic Envs preferentially replicated in peripheral blood mononuclear cells (PBMC) and a CD4 T-cell line compared to monocyte-derived macrophages, or dendritic cells (DC). In a model of\n            <jats:italic>trans<\/jats:italic>\n            infection from monocyte-derived dendritic cells to T cells, chimeric virus from acute Envs achieved significantly lower titers compared to chronic Envs. Challenge of primary human mucosal tissues revealed significantly higher levels of replication in chronic Env-expressing virus in rectal tissue compared to cervical and penile tissues and enhanced replication in tonsillar tissue relative to acute Envs. In agreement with data from the DC to T-cell\n            <jats:italic>trans<\/jats:italic>\n            infection assay, chronic Env-chimeric virus pools were transmitted more efficiently by migratory cells from cervical and penile tissues to CD4\n            <jats:sup>+<\/jats:sup>\n            T cells than individual acute Env chimeras. These data indicate that virus with HIV-1 Envs of transmitted acute infections preferentially replicate in T cells rather than macrophages or dendritic cells and are less efficiently transmitted from antigen-presenting cells to CD4 T cells than chronic Envs. Such properties together with chemokine (C-C motif) receptor 5 (CCR5) use may confer an advantage for transmission.\n          <\/jats:p>","DOI":"10.1128\/jvi.02216-12","type":"journal-article","created":{"date-parts":[[2012,11,8]],"date-time":"2012-11-08T07:14:14Z","timestamp":1352358854000},"page":"890-899","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":27,"title":["Mucosal Tissue Tropism and Dissemination of HIV-1 Subtype B Acute Envelope-Expressing Chimeric Virus"],"prefix":"10.1128","volume":"87","author":[{"given":"Deborah F. L.","family":"King","sequence":"first","affiliation":[{"name":"Centre for Infection, Clinical Sciences, St. George's University of London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asna A.","family":"Siddiqui","sequence":"additional","affiliation":[{"name":"Centre for Infection, Clinical Sciences, St. George's University of London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviana","family":"Buffa","sequence":"additional","affiliation":[{"name":"Centre for Infection, Clinical Sciences, St. George's University of London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Fischetti","sequence":"additional","affiliation":[{"name":"Centre for Infection, Clinical Sciences, St. George's University of London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong","family":"Gao","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Stieh","sequence":"additional","affiliation":[{"name":"Centre for Infection, Clinical Sciences, St. George's University of London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul F.","family":"McKay","sequence":"additional","affiliation":[{"name":"Centre for Infection, Clinical Sciences, St. George's University of London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Rogers","sequence":"additional","affiliation":[{"name":"Centre for Infection, Clinical Sciences, St. George's University of London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Ochsenbauer","sequence":"additional","affiliation":[{"name":"Department of Medicine and UAB CFAR, University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John C.","family":"Kappes","sequence":"additional","affiliation":[{"name":"Department of Medicine and UAB CFAR, University of Alabama at Birmingham, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric J.","family":"Arts","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Department of Medicine, Case Western Reserve University, Cleveland, Ohio, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin J.","family":"Shattock","sequence":"additional","affiliation":[{"name":"Centre for Infection, Clinical Sciences, St. George's University of London, London, United 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