{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:05:11Z","timestamp":1790622311673,"version":"4.1.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2018,6,15]],"date-time":"2018-06-15T00:00:00Z","timestamp":1529020800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Fondation pour l'innovation en infectiologie"},{"DOI":"10.13039\/501100018873","name":"LABEX ECOFECT","doi-asserted-by":"crossref","award":["ANR-11-LBX-0048"],"award-info":[{"award-number":["ANR-11-LBX-0048"]}],"id":[{"id":"10.13039\/501100018873","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR-11-BSV_019-02"],"award-info":[{"award-number":["ANR-11-BSV_019-02"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002915","name":"Fondation pour la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["Equipe FRM"],"award-info":[{"award-number":["Equipe FRM"]}],"id":[{"id":"10.13039\/501100002915","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2018,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Several Old World and New World arenaviruses are responsible for severe endemic and epidemic hemorrhagic fevers, whereas other members of the\n                    <jats:named-content content-type=\"genus-species\">Arenaviridae<\/jats:named-content>\n                    family are nonpathogenic. To date, no approved vaccines, antivirals, or specific treatments are available, except for Jun\u00edn virus. However, protection of nonhuman primates against Lassa fever virus (LASV) is possible through the inoculation of the closely related but nonpathogenic Mopeia virus (MOPV) before challenge with LASV. We reasoned that this virus, modified by using reverse genetics, would represent the basis for the generation of a vaccine platform against LASV and other pathogenic arenaviruses. After showing evidence of exoribonuclease (ExoN) activity in NP of MOPV, we found that this activity was essential for multiplication in antigen-presenting cells. The introduction of multiple mutations in the ExoN site of MOPV NP generated a hyperattenuated strain (MOPV\n                    <jats:sub>ExoN6b<\/jats:sub>\n                    ) that is (i) genetically stable over passages, (ii) has increased immunogenic properties compared to those of MOPV, and (iii) still promotes a strong type I interferon (IFN) response. MOPV\n                    <jats:sub>ExoN6b<\/jats:sub>\n                    was further modified to harbor the envelope glycoproteins of heterologous pathogenic arenaviruses, such as LASV or Lujo, Machupo, Guanarito, Chapare, or Sabia virus in order to broaden specific antigenicity while preserving the hyperattenuated characteristics of the parental strain. Our MOPV-based vaccine candidate for LASV, MOPEVAC\n                    <jats:sub>LASV<\/jats:sub>\n                    , was used in a one-shot immunization assay in nonhuman primates and fully protected them from a lethal challenge with LASV. Thus, our hyperattenuated strain of MOPV constitutes a promising new live-attenuated vaccine platform to immunize against several, if not all, pathogenic arenaviruses.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    Arenaviruses are emerging pathogens transmitted to humans by rodents and responsible for endemic and epidemic hemorrhagic fevers of global concern. Nonspecific symptoms associated with the onset of infection make these viruses difficult to distinguish from other endemic pathogens. Moreover, the unavailability of rapid diagnosis in the field delays the identification of the virus and early care for treatment and favors spreading. The vaccination of exposed populations would be of great help to decrease morbidity and human-to-human transmission. Using reverse genetics, we generated a vaccine platform for pathogenic arenaviruses based on a modified and hyperattenuated strain of the nonpathogenic Mopeia virus and showed that the Lassa virus candidate fully protected nonhuman primates from a lethal challenge. These results showed that a rationally designed recombinant MOPV-based vaccine is safe, immunogenic, and efficacious in nonhuman primates.\n                  <\/jats:p>","DOI":"10.1128\/jvi.02230-17","type":"journal-article","created":{"date-parts":[[2018,3,28]],"date-time":"2018-03-28T06:42:13Z","timestamp":1522219333000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":58,"title":["A Vaccine Platform against Arenaviruses Based on a Recombinant Hyperattenuated Mopeia Virus Expressing Heterologous Glycoproteins"],"prefix":"10.1128","volume":"92","author":[{"given":"Xavier","family":"Carnec","sequence":"first","affiliation":[{"name":"Unit\u00e9 de Biologie des Infections Virales Emergentes, Institut Pasteur, Centre International de Recherche en Infectiologie (INSERM, CNRS, ENS Lyon, Universit\u00e9 Lyon I), Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu","family":"Mateo","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biologie des Infections Virales Emergentes, Institut Pasteur, Centre International de Recherche en Infectiologie (INSERM, CNRS, ENS Lyon, Universit\u00e9 Lyon I), Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Page","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biologie des Infections Virales Emergentes, Institut Pasteur, Centre International de Recherche en Infectiologie (INSERM, CNRS, ENS Lyon, Universit\u00e9 Lyon I), Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Reynard","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biologie des Infections Virales Emergentes, Institut Pasteur, Centre International de Recherche en Infectiologie (INSERM, CNRS, ENS Lyon, Universit\u00e9 Lyon I), Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jimmy","family":"Hortion","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biologie des Infections Virales Emergentes, Institut Pasteur, Centre International de Recherche en Infectiologie (INSERM, CNRS, ENS Lyon, Universit\u00e9 Lyon I), Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Picard","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biologie des Infections Virales Emergentes, Institut Pasteur, Centre International de Recherche en Infectiologie (INSERM, CNRS, ENS Lyon, Universit\u00e9 Lyon I), Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elsie","family":"Yekwa","sequence":"additional","affiliation":[{"name":"CNRS, Architecture et Fonction des Macromol\u00e9cules Biologiques UMR 7257, Aix-Marseille Universit\u00e9, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Barrot","sequence":"additional","affiliation":[{"name":"Laboratoire P4 Jean M\u00e9rieux\u2014INSERM, US003, INSERM, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Barron","sequence":"additional","affiliation":[{"name":"Laboratoire P4 Jean M\u00e9rieux\u2014INSERM, US003, INSERM, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Vallve","sequence":"additional","affiliation":[{"name":"Laboratoire P4 Jean M\u00e9rieux\u2014INSERM, US003, INSERM, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herv\u00e9","family":"Raoul","sequence":"additional","affiliation":[{"name":"Laboratoire P4 Jean M\u00e9rieux\u2014INSERM, US003, INSERM, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Carbonnelle","sequence":"additional","affiliation":[{"name":"Laboratoire P4 Jean M\u00e9rieux\u2014INSERM, US003, INSERM, Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Ferron","sequence":"additional","affiliation":[{"name":"CNRS, Architecture et Fonction des Macromol\u00e9cules Biologiques UMR 7257, Aix-Marseille Universit\u00e9, Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvain","family":"Baize","sequence":"additional","affiliation":[{"name":"Unit\u00e9 de Biologie des Infections Virales Emergentes, Institut Pasteur, Centre International de Recherche en Infectiologie (INSERM, CNRS, ENS Lyon, Universit\u00e9 Lyon I), Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/155.3.437"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1990.03450160063030"},{"key":"e_1_3_2_4_2","first-page":"1791","volume-title":"Fields virology","author":"Buchmeier MJ","year":"2007","unstructured":"Buchmeier MJ, de la Torre J-C, Peters CJ. 2007. 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