{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T16:01:36Z","timestamp":1787068896249,"version":"build-2736575974"},"reference-count":14,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2007,6,15]],"date-time":"2007-06-15T00:00:00Z","timestamp":1181865600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2007,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>A 190-nucleotide (nt) packaging signal (PS) located in the 3\u2032 end of open reading frame 1b in the mouse hepatitis virus, a group IIa coronavirus, was previously postulated to direct genome RNA packaging. Based on phylogenetic data and structure probing, we have identified a 95-nt hairpin within the 190-nt PS domain which is conserved in all group IIa coronaviruses but not in the severe acute respiratory syndrome coronavirus (group IIb), group I coronaviruses, or group III coronaviruses. The hairpin is composed of six copies of a repeating structural subunit that consists of 2-nt bulges and 5-bp stems. We propose that repeating AA bulges are characteristic features of group IIa PSs.<\/jats:p>","DOI":"10.1128\/jvi.02231-06","type":"journal-article","created":{"date-parts":[[2007,4,11]],"date-time":"2007-04-11T20:25:56Z","timestamp":1176323156000},"page":"6771-6774","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["New Structure Model for the Packaging Signal in the Genome of Group IIa Coronaviruses"],"prefix":"10.1128","volume":"81","author":[{"given":"Shih-Cheng","family":"Chen","sequence":"first","affiliation":[{"name":"Leiden Institute of Chemistry, Leiden University, P.O. Box 9502, 2300 RA Leiden, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erwin","family":"van den Born","sequence":"additional","affiliation":[{"name":"Molecular Virology Laboratory, Department of Medical Microbiology, Leiden University Medical Center, P.O. 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