{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:43:52Z","timestamp":1791308632472,"version":"4.3.1"},"reference-count":72,"publisher":"American Society for Microbiology","issue":"24","license":[{"start":{"date-parts":[[2013,12,15]],"date-time":"2013-12-15T00:00:00Z","timestamp":1387065600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2013,12,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The human JC polyomavirus (JCPyV) causes the rapidly progressing demyelinating disease progressive multifocal leukoencephalopathy (PML). The disease occurs most often in individuals with AIDS but also occurs in individuals receiving immunomodulatory therapies for immune-related diseases such as multiple sclerosis. JCPyV infection of host cells requires the pentasaccharide lactoseries tetrasaccharide c (LSTc) and the serotonin receptor 5-hydroxytryptamine (5-HT) receptor 5-HT<jats:sub>2A<\/jats:sub>R. While LSTc is involved in the initial attachment of virus to cells via interactions with VP1, the mechanism by which 5-HT<jats:sub>2A<\/jats:sub>R contributes to infection is not clear. To further define the roles of serotonin receptors in infection, HEK293A cells, which are poorly permissive to JCPyV, were transfected with 14 different isoforms of serotonin receptor. Only 5-HT<jats:sub>2<\/jats:sub>receptors were found to support infection by JCPyV. None of the other 11 isoforms of serotonin receptor supported JCPyV infection. Expression of 5-HT<jats:sub>2<\/jats:sub>receptors did not increase binding of JCPyV to cells, but this was not unexpected, given that the cells uniformly expressed the major attachment receptor, LSTc. Infection of these cells remained sensitive to inhibition with soluble LSTc, confirming that LSTc recognition is required for JCPyV infection. Virus internalization into HEK293A cells was significantly and specifically enhanced when 5HT<jats:sub>2<\/jats:sub>receptors were expressed. Taken together, these data confirm that the carbohydrate LSTc is the attachment receptor for JCPyV and that the type 2 serotonin receptors contribute to JCPyV infection by facilitating entry.<\/jats:p>","DOI":"10.1128\/jvi.02252-13","type":"journal-article","created":{"date-parts":[[2013,10,3]],"date-time":"2013-10-03T00:59:30Z","timestamp":1380761970000},"page":"13490-13498","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":81,"title":["5-HT<sub>2<\/sub>Receptors Facilitate JC Polyomavirus Entry"],"prefix":"10.1128","volume":"87","author":[{"given":"Benedetta","family":"Assetta","sequence":"first","affiliation":[{"name":"Graduate Program in Pathobiology, Brown University, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melissa S.","family":"Maginnis","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Gracia Ahufinger","sequence":"additional","affiliation":[{"name":"UGC Microbiology, IMIBIC-University Hospital Reina Sof\u00eda-University of C\u00f3rdoba, C\u00f3rdoba, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheila A.","family":"Haley","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gretchen V.","family":"Gee","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian D. S.","family":"Nelson","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bethany A.","family":"O'Hara","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacy-ann A.","family":"Allen Ramdial","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walter J.","family":"Atwood","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, Providence, Rhode Island, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/597126"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.05175-11"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1080\/13550280260422758"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.27.6.1174-1179.1989"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/142.1.1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(91)90069-N"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.63.10.4445-4449.1989"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/167.1.13"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02302-08"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2009.03.020"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.2134\/jeq2010.0062"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198802043180507"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490270405"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.70.10.7004-7012.1996"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/504947"},{"key":"e_1_3_2_17_2","first-page":"115","article-title":"JC virus infection in cells from lymphoid tissue","volume":"94","author":"Monaco MC","year":"1998","unstructured":"MonacoMCShinJMajorEO. 1998. JC virus infection in cells from lymphoid tissue. Dev. Biol. Stand. 94:115\u2013122.","journal-title":"Dev. Biol. Stand."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/653823"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00609-10"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.21443"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(71)91777-6"},{"key":"e_1_3_2_22_2","first-page":"299","article-title":"Papovavirus antigens in paraffin sections of PML brains","volume":"105","author":"Budka H","year":"1983","unstructured":"BudkaHShahKV. 1983. Papovavirus antigens in paraffin sections of PML brains. Prog. Clin. Biol. Res. 105:299\u2013309.","journal-title":"Prog. Clin. Biol. Res."},{"key":"e_1_3_2_23_2","first-page":"495","article-title":"The pathogenesis of progressive multifocal leukoencephalopathy","volume":"12","author":"Berger JR","year":"2011","unstructured":"BergerJRKhaliliK. 2011. The pathogenesis of progressive multifocal leukoencephalopathy. Discov. Med. 12:495\u2013503.","journal-title":"Discov. Med."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05031-11"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.080708.082655"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-10-186999"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa051782"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1159\/000109933"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/ana.23893"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11910-012-0313-4"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1097\/WCO.0b013e328346be57"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.3949\/ccjm.78.s2.03"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrneurol.2010.164"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2010.09.004"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103492"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00978-10"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2007.02.018"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2007.11.002"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.2165\/00023210-200923050-00006"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.neuroscience.2012.11.047"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/syn.890180310"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1986.tb04590.x"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1159\/000333780"},{"key":"e_1_3_2_44_2","first-page":"35","article-title":"Mirtazapine in an HIV-1 infected patient with progressive multifocal leukoencephalopathy","volume":"17","author":"Lanzafame M","year":"2009","unstructured":"LanzafameMFerrariSLattuadaECorsiniFDeganelloRVentoSConciaE. 2009. Mirtazapine in an HIV-1 infected patient with progressive multifocal leukoencephalopathy. Infez. Med. 17:35\u201337.","journal-title":"Infez. Med."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1001\/archneurol.2008.557"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1086\/520514"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13365-013-0167-9"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-006-0286-1"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0028-3908(97)00036-1"},{"key":"e_1_3_2_50_2","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1016\/S0026-895X(25)10488-4","article-title":"Molecular cloning of human 5-hydroxytryptamine3 receptor: heterogeneity in distribution and function among species","volume":"48","author":"Miyake A","year":"1995","unstructured":"MiyakeAMochizukiSTakemotoYAkuzawaS. 1995. Molecular cloning of human 5-hydroxytryptamine3 receptor: heterogeneity in distribution and function among species. Mol. Pharmacol. 48:407\u2013416.","journal-title":"Mol. Pharmacol."},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.3109\/13550289809113481"},{"key":"e_1_3_2_52_2","first-page":"9","article-title":"Propagation and assay of the JC virus","volume":"165","author":"Liu CK","year":"2001","unstructured":"LiuCKAtwoodWJ. 2001. Propagation and assay of the JC virus. Methods Mol. Biol. 165:9\u201317.","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02179-10"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.5.2288-2292.2000"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.80.6.2760-2770.2006"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.69.1.531"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055799"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2013.05.005"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003244"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00038-09"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00231-8"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00330-13"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.3.1565-1570.2001"},{"key":"e_1_3_2_64_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.24.12961-12967.2003"},{"key":"e_1_3_2_65_2","first-page":"105","volume-title":"Serotonin receptors in neurobiology","author":"Raote I","year":"2007","unstructured":"RaoteIBhattacharyaAPanickerMM. 2007. Serotonin 2A (5-HT2A) receptor function: ligand-dependent mechanisms and pathways, p 105\u2013132. In AChattopadhyay (ed), Serotonin receptors in neurobiology. CRC Press, Boca Raton, FL."},{"key":"e_1_3_2_66_2","doi-asserted-by":"publisher","DOI":"10.1124\/mol.112.078626"},{"key":"e_1_3_2_67_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.1.250-256.2004"},{"key":"e_1_3_2_68_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1460-9568.2006.05023.x"},{"key":"e_1_3_2_69_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2007.06.059"},{"key":"e_1_3_2_70_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1995.tb14977.x"},{"key":"e_1_3_2_71_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smrv.2010.11.003"},{"key":"e_1_3_2_72_2","doi-asserted-by":"publisher","DOI":"10.1192\/bjp.bp.109.067710"},{"key":"e_1_3_2_73_2","doi-asserted-by":"publisher","DOI":"10.4067\/S0716-10182012000200016"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02252-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02252-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,30]],"date-time":"2025-04-30T15:23:38Z","timestamp":1746026618000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.02252-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12,15]]},"references-count":72,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2013,12,15]]}},"alternative-id":["10.1128\/JVI.02252-13"],"URL":"https:\/\/doi.org\/10.1128\/jvi.02252-13","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12,15]]},"assertion":[{"value":"2013-08-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-27","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}