{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,9,13]],"date-time":"2023-09-13T18:57:43Z","timestamp":1694631463275},"reference-count":29,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2008,4,15]],"date-time":"2008-04-15T00:00:00Z","timestamp":1208217600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2008,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The HA2 protein of the\n            <jats:italic>Helicoverpa armigera<\/jats:italic>\n            single-nucleocapsid nucleopolyhedrovirus (HearNPV) is a WASP homology protein capable of nucleating branched actin filaments in the presence of the Arp2\/3 complex in vitro. To determine the role of\n            <jats:italic>ha2<\/jats:italic>\n            in the HearNPV life cycle,\n            <jats:italic>ha2<\/jats:italic>\n            knockout and\n            <jats:italic>ha2<\/jats:italic>\n            repair bacmids were constructed. Transfection and infection analysis demonstrated that the\n            <jats:italic>ha2<\/jats:italic>\n            null bacmid was unable to produce infectious budded virus (BV), while the repair bacmid rescued the defect. In vitro analysis demonstrated that the WCA domain of HA2 accelerates Arp2\/3-mediated actin assembly and is indispensable to the function of HA2. However, analysis of the repaired recombinant with a series of truncated\n            <jats:italic>ha2<\/jats:italic>\n            mutants demonstrated that the WCA domain was essential but not enough to yield infectious virions, and a hydrophobic domain (H domain) consisting of amino acids (aa) 167 to 193 played a pivotal role in the production of BV. Subcellular localization analysis with enhanced green fluorescent protein fusions showed that the H domain functioned as a nuclear localization signal. In addition, deletion of the C terminus of the\n            <jats:italic>ha2<\/jats:italic>\n            product, a phosphatidylinositol 4-kinase homolog, dramatically decreased the viral titer, while deletion of 128 aa from the N terminus did not affect HA2 function.\n          <\/jats:p>","DOI":"10.1128\/jvi.02319-07","type":"journal-article","created":{"date-parts":[[2008,1,31]],"date-time":"2008-01-31T01:46:47Z","timestamp":1201744007000},"page":"4072-4081","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Identification of a Hydrophobic Domain of HA2 Essential to Morphogenesis of\n            <i>Helicoverpa armigera<\/i>\n            Nucleopolyhedrovirus"],"prefix":"10.1128","volume":"82","author":[{"given":"Qian","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071"},{"name":"Department of Biochemistry &amp; Molecular Biology, Nanjing Medical University, Nanjing, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Changyong","family":"Liang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhua","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinwen","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00168-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0402727101"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.65.3.1219-1227.1991"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-82-1-241"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1006\/jipa.2000.4963"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.120163297"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.8.3771-3778.2001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.4.595-626.2001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1133348"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.8.4050"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.2144\/02324st04"},{"key":"e_1_3_2_13_2","first-page":"1881","volume":"81","year":"2000","unstructured":"Kasman, L. 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