{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:50:06Z","timestamp":1791208206234,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2010,6,15]],"date-time":"2010-06-15T00:00:00Z","timestamp":1276560000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2010,6,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>TRIM5 proteins mediate a potent block to the cross-species transmission of retroviruses, the most well known being the TRIM5\u03b1 protein from rhesus macaques, which potently inhibits human immunodeficiency virus type 1 (HIV-1) infection. This restriction occurs at an early stage in the replication cycle and is mediated by the binding of TRIM5 proteins to determinants present in the retroviral capsid. TRIM5\u03b1, as well as other TRIM family proteins, has been shown to be regulated by interferons (IFN). Here we show that TRIM5\u03b1 associates with another IFN-induced gene, sequestosome-1\/p62 (p62). p62 plays a role in several signal transduction cascades that are important for maintaining the antiviral state of cells. Here we demonstrate that p62 localizes to both human and rhesus macaque TRIM5\u03b1 cytoplasmic bodies, and fluorescence resonance energy transfer (FRET) analysis demonstrates that these proteins closely associate in these structures. When p62 expression was knocked down via small interfering RNA (siRNA), the number of TRIM5\u03b1 cytoplasmic bodies and the level of TRIM5\u03b1 protein expression were reduced in cell lines stably expressing epitope-tagged versions of TRIM5\u03b1. In accordance with these data, p62 knockdown resulted in reduced TRIM5\u03b1-mediated retroviral restriction in cells expressing epitope-tagged TRIM5\u03b1 or expressing endogenously expressed human TRIM5\u03b1. p62 may therefore operate to enhance TRIM5\u03b1-mediated retroviral restriction, contributing to the antiviral state of cells following IFN treatment.<\/jats:p>","DOI":"10.1128\/jvi.02412-09","type":"journal-article","created":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T02:45:32Z","timestamp":1270089932000},"page":"5997-6006","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["p62\/Sequestosome-1 Associates with and Sustains the Expression of Retroviral Restriction Factor TRIM5\u03b1"],"prefix":"10.1128","volume":"84","author":[{"given":"Christopher","family":"O'Connor","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Pertel","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth","family":"Gray","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seth L.","family":"Robia","sequence":"additional","affiliation":[{"name":"Department of Physiology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna C.","family":"Bakowska","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeremy","family":"Luban","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Molecular Medicine, University of Geneva, Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward M.","family":"Campbell","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Stritch School of Medicine, Loyola University Chicago, Maywood, Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01052-06"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.21787"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2005.10.173"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000007"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.047357"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.e06-12-1075"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.11.5745-5755.2004"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200706154"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0004894"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-5-59"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00126-4"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00703-07"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2005.12.040"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.006288-0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05732"},{"key":"e_1_3_2_17_2","first-page":"424","volume":"50","year":"2009","unstructured":"Gao, B., Z. 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