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The majority of the isolates (13\/18) induced severe morbidity and mortality in DBA\/2 mice upon intranasal challenge with 1 million infectious doses. Also, at a 100-fold-lower dose, more than 50% of the viruses induced severe weight loss, and mice succumbed to the infection. In contrast, only two virus strains were pathogenic for C57BL\/6 mice upon high-dose inoculation. Therefore, DBA\/2 mice are a suitable model to validate influenza A virus vaccines and antiviral therapies without the need for extensive viral adaptation. Correspondingly, we used the DBA\/2 model to assess the level of protection afforded by preexisting pandemic H1N1 2009 virus (H1N1pdm) cross-reactive human antibodies detected by a hemagglutination inhibition assay. Passive transfer of these antibodies prior to infection protected mice from H1N1pdm-induced pathogenicity, demonstrating the effectiveness of these cross-reactive neutralizing antibodies<jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1128\/jvi.02444-09","type":"journal-article","created":{"date-parts":[[2010,5,20]],"date-time":"2010-05-20T02:14:39Z","timestamp":1274321679000},"page":"7662-7667","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Cross-Reactive Neutralizing Antibodies Directed against Pandemic H1N1 2009 Virus Are Protective in a Highly Sensitive DBA\/2 Mouse Influenza Model"],"prefix":"10.1128","volume":"84","author":[{"given":"Adrianus C. 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