{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:04:41Z","timestamp":1791086681851,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2011,5,1]],"date-time":"2011-05-01T00:00:00Z","timestamp":1304208000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The prion agent is the infectious particle causing spongiform encephalopathies in animals and humans and is thought to consist of an altered conformation (PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    ) of the normal and ubiquitous prion protein PrP\n                    <jats:sup>C<\/jats:sup>\n                    . The interaction of the prion agent with the immune system, particularly the humoral immune response, has remained unresolved. Here we investigated the immunogenicity of full-length native and infectious prions, as well as the specific biological effects of the resulting monoclonal antibodies (MAbs) on the binding and clearance of prions in cell culture and in\n                    <jats:named-content content-type=\"genus-species\">in vivo<\/jats:named-content>\n                    therapy. Immunization of prion knockout (\n                    <jats:italic>\n                      Prnp\n                      <jats:sup>0\/0<\/jats:sup>\n                    <\/jats:italic>\n                    ) mice with phosphotungstic acid-purified mouse prions resulted in PrP-specific monoclonal antibodies with binding specificities selective for PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    or for both PrP\n                    <jats:sup>C<\/jats:sup>\n                    and PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    . PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    -specific MAb W261, of the IgG1 isotype, reacted with prions from mice, sheep with scrapie, deer with chronic wasting disease (CWD), and humans with sporadic and variant Creutzfeldt-Jakob disease (CJD) in assays including a capture enzyme-linked immunosorbent assay (ELISA) system. This PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    -specific antibody was unable to clear prions from mouse neuroblastoma cells (ScN2a) permanently infected with scrapie, whereas the high-affinity MAb W226, recognizing both isoforms, PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    and PrP\n                    <jats:sup>C<\/jats:sup>\n                    , did clear prions from ScN2a cells, as determined by a bioassay. However, an attempt to treat intraperitoneally prion infected mice with full-length W226 or with a recombinant variable-chain fragment (scFv) from W226 could only slightly delay the incubation time. We conclude that (i) native, full-length PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    elicits a prion-specific antibody response in PrP knockout mice, (ii) a PrP\n                    <jats:sup>Sc<\/jats:sup>\n                    -specific antibody had no prion-clearing effect, and (iii) even a high-affinity MAb that clears prions\n                    <jats:named-content content-type=\"genus-species\">in vitro<\/jats:named-content>\n                    (W226) may not necessarily protect against prion infection, contrary to previous reports using different antibodies.\n                  <\/jats:p>","DOI":"10.1128\/jvi.02467-10","type":"journal-article","created":{"date-parts":[[2011,2,23]],"date-time":"2011-02-23T23:23:04Z","timestamp":1298503384000},"page":"4538-4546","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Biological Effects and Use of PrP\n                    <sup>Sc<\/sup>\n                    - and PrP-Specific Antibodies Generated by Immunization with Purified Full-Length Native Mouse Prions"],"prefix":"10.1128","volume":"85","author":[{"given":"Benjamin","family":"Petsch","sequence":"first","affiliation":[{"name":"Friedrich-Loeffler-Institut, Institute of Immunology, Tuebingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"M\u00fcller-Schiffmann","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Heinrich Heine University D\u00fcsseldorf, D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Lehle","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Institute of Immunology, Tuebingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeta","family":"Zirdum","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Institute of Immunology, Tuebingen"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingrid","family":"Prikulis","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Heinrich Heine University D\u00fcsseldorf, D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franziska","family":"Kuhn","sequence":"additional","affiliation":[{"name":"Prionics AG, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex J.","family":"Raeber","sequence":"additional","affiliation":[{"name":"Prionics AG, Schlieren, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James W.","family":"Ironside","sequence":"additional","affiliation":[{"name":"National Creutzfeldt-Jakob Disease Surveillance Unit, University of Edinburgh, Edinburgh, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carsten","family":"Korth","sequence":"additional","affiliation":[{"name":"Department of Neuropathology, Heinrich Heine University D\u00fcsseldorf, D\u00fcsseldorf, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lothar","family":"Stitz","sequence":"additional","affiliation":[{"name":"Friedrich-Loeffler-Institut, Institute of Immunology, Tuebingen"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3050"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jneuroim.2003.08.036"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830251236"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.15.1.235"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90662-8"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M402270200"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05306.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6815801"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.74.9.4377-4386.2000"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/356577a0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0702662104"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh340"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.151242598"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1996.tb00467.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1000673"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-79-4-937"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.15.7097-7106.2001"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/256495a0"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.161274798"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/36337"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(96)00165-2"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1656(99)00115-7"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1100195"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409079102"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90207-6"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403522101"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nbd.2006.06.008"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2008.07.023"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1089\/153685903322328992"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90333-2"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.6.1750-1755.1998"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560011014"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm883"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/35089090"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003872"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6801762"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.22.10608"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3987"},{"key":"e_1_3_2_40_2","first-page":"493","article-title":"A simple method of estimating fifty percent endpoints","volume":"27","author":"Reed L.","year":"1938","unstructured":"ReedL. MuenchH.. 1938. A simple method of estimating fifty percent endpoints. Am. J. Hyg. 27:493\u2013497.","journal-title":"Am. J. Hyg."},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/2654"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.16.10796-10806.2005"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M610051200"},{"key":"e_1_3_2_44_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj20021280"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(01)05403-4"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature01457"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1711.2005.01384.x"}],"container-title":["Journal of Virology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02467-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JVI.02467-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:28:26Z","timestamp":1646479706000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JVI.02467-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,5]]},"references-count":47,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2011,5]]}},"alternative-id":["10.1128\/JVI.02467-10"],"URL":"https:\/\/doi.org\/10.1128\/jvi.02467-10","relation":{},"ISSN":["0022-538X","1098-5514"],"issn-type":[{"value":"0022-538X","type":"print"},{"value":"1098-5514","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,5]]},"assertion":[{"value":"2010-11-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-02-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-04-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}