{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T10:56:32Z","timestamp":1791456992954,"version":"4.3.4"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2009,4,15]],"date-time":"2009-04-15T00:00:00Z","timestamp":1239753600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Virol"],"published-print":{"date-parts":[[2009,4,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rift Valley fever virus (RVFV) is a member of the genus\n            <jats:italic>Phlebovirus<\/jats:italic>\n            within the family\n            <jats:italic>Bunyaviridae<\/jats:italic>\n            . It is a mosquito-borne zoonotic agent that can cause hemorrhagic fever in humans. The enveloped RVFV virions are known to be covered by capsomers of the glycoproteins G\n            <jats:sub>N<\/jats:sub>\n            and G\n            <jats:sub>C<\/jats:sub>\n            , organized on a T=12 icosahedral lattice. However, the structural units forming the RVFV capsomers have not been determined. Conflicting biochemical results for another phlebovirus (Uukuniemi virus) have indicated the existence of either G\n            <jats:sub>N<\/jats:sub>\n            and G\n            <jats:sub>C<\/jats:sub>\n            homodimers or G\n            <jats:sub>N<\/jats:sub>\n            -G\n            <jats:sub>C<\/jats:sub>\n            heterodimers in virions. Here, we have studied the structure of RVFV using electron cryo-microscopy combined with three-dimensional reconstruction and single-particle averaging. The reconstruction at 2.2-nm resolution revealed the organization of the glycoprotein shell, the lipid bilayer, and a layer of ribonucleoprotein (RNP). Five- and six-coordinated capsomers are formed by the same basic structural unit. Molecular-mass measurements suggest a G\n            <jats:sub>N<\/jats:sub>\n            -G\n            <jats:sub>C<\/jats:sub>\n            heterodimer as the most likely candidate for this structural unit. Both leaflets of the lipid bilayer were discernible, and the glycoprotein transmembrane densities were seen to modulate the curvature of the lipid bilayer. RNP densities were situated directly underneath the transmembrane densities, suggesting an interaction between the glycoprotein cytoplasmic tails and the RNPs. The success of the single-particle averaging approach taken in this study suggests that it is applicable in the study of other phleboviruses, as well, enabling higher-resolution description of these medically important pathogens.\n          <\/jats:p>","DOI":"10.1128\/jvi.02483-08","type":"journal-article","created":{"date-parts":[[2009,2,5]],"date-time":"2009-02-05T02:10:57Z","timestamp":1233799857000},"page":"3762-3769","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":124,"title":["Electron Cryo-Microscopy and Single-Particle Averaging of Rift Valley Fever Virus: Evidence for G\n            <sub>N<\/sub>\n            -G\n            <sub>C<\/sub>\n            Glycoprotein Heterodimers"],"prefix":"10.1128","volume":"83","author":[{"given":"Juha T.","family":"Huiskonen","sequence":"first","affiliation":[{"name":"Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany"},{"name":"Institute of Biotechnology, University of Helsinki, P.O. Box 65, FI-00014 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna K.","family":"O\u0308verby","sequence":"additional","affiliation":[{"name":"Department of Virology, University of Freiburg, Hermann-Herder-Strasse 11, D-79008 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedemann","family":"Weber","sequence":"additional","affiliation":[{"name":"Department of Virology, University of Freiburg, Hermann-Herder-Strasse 11, D-79008 Freiburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kay","family":"Gru\u0308newald","sequence":"additional","affiliation":[{"name":"Department of Molecular Structural Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18, D-82152 Martinsried, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/jsbi.1996.0020"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(02)00295-9"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01316748"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02095-06"},{"key":"e_1_3_2_6_2","first-page":"73","volume":"56","year":"2007","unstructured":"Centers for Disease Control and Prevention. 2007. 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